Fig. 3.

Effects of cell passaging on cell migration of HUVECs. a Representative confocal DIC images show the scratch (the first image) and the cells migrating into the scratch at passages 1, 5, 10, 15, 20, 25, 30, and 35 (P1, P5, P10, P15, P20, P25, P30, and P35, respectively). b The average number of migrating cells per field (the dashed rectangle indicates a field in the second image of the upper panel of a). Values are expressed as mean ± SEM (n ≥ 6 from three independent experiments; asterisk, P < 0.05)