Skip to main content
. 2014 Jan 21;2014:187071. doi: 10.1155/2014/187071

Figure 2.

Figure 2

Effect of CDP-choline on cardiac myocytes survival. (a) Cardiac myocyte cultures were treated with increasing concentrations of CDP-choline as indicated. The proportion of viable cells was determined at 24 hrs using the MTT assay. (b) Effect of CDP-choline preconditioning. Cardiac myocytes were coverslipped for 2 hrs. The coverslip was then removed, and the cells were reperfused. CDP-choline was added to coverslipped cells 1 hr prior to reperfusion at the indicated concentrations. Cell viability was assessed at 24 hrs using the MTT assay. (c) Effect of CDP-choline postconditioning. Cardiac myocytes were coverslipped for 2 hrs. The coverslip was then removed, and the cells were reperfused. CDP-choline was added 1 hr after reperfusion. The proportion of viable cells was assessed at 24 hrs using the MTT assay. All values reported represent the average of three independent tests. Error bars indicate the standard error of the mean. *P < 0.05 versus untreated control values.