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Analysis of signaling pathways in MCN. Analysis of transforming growth factor–βsignaling in MCN lesions by immunostaining for the nuclear translocation of phosphorylated Smad2/3 (A) and immunostaining for Smad4 (B). Assessment of active Notch signaling via immunostaining for Hes1 (C). Immunostaining for the pancreas progenitor marker Pdx1 (D).
