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. 1984 Nov;81(21):6603–6607. doi: 10.1073/pnas.81.21.6603

Amino acid sequence of bovine heart coupling factor 6.

J K Fang, J W Jacobs, B I Kanner, E Racker, R A Bradshaw
PMCID: PMC391978  PMID: 6149548

Abstract

The amino acid sequence of bovine heart mitochondrial coupling factor 6 (F6) has been determined by automated Edman degradation of the whole protein and derived peptides. Preparations based on heat precipitation and ethanol extraction showed allotypic variation at three positions while material further purified by HPLC yielded only one sequence that also differed by a Phe-Thr replacement at residue 62. The mature protein contains 76 amino acids with a calculated molecular weight of 9006 and a pI of approximately equal to 5, in good agreement with experimentally measured values. The charged amino acids are mainly clustered at the termini and in one section in the middle; these three polar segments are separated by two segments relatively rich in nonpolar residues. Chou-Fasman analysis suggests three stretches of alpha-helix coinciding (or within) the high-charge-density sequences with a single beta-turn at the first polar-nonpolar junction. Comparison of the F6 sequence with those of other proteins did not reveal any homologous structures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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