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. 1984 Nov;81(21):6866–6870. doi: 10.1073/pnas.81.21.6866

Isolation and sequencing of two cerebellum-specific peptides

J Randall Slemmon *, Russell Blacher †, Waleed Danho ‡, James L Hempstead *, James I Morgan *,§
PMCID: PMC392033  PMID: 16593526

Abstract

By comparing the HPLC elution profiles of peptides isolated from different brain regions, two cerebellum-specific species have been identified. The peptides were isolated by sequential chromatography on reverse phase, followed by ion-pairing HPLC using alkane sulfates as pairing reagents. The sequences of both peptides have been determined by gasphase Edman degradation and carboxypeptidase Y analysis and were confirmed by synthesis. The larger peptide, termed cerebellin, is a hexadecamer of primary amino acid sequence NH2-Ser-Gly-Ser-Ala-Lys-Val-Ala-Phe-Ser-Ala-Ile-Arg-Ser-Thr-Asn-His- OH. The second peptide is a pentadecamer with a primary sequence identical to residues 2-16 of cerebellin, the nomenclature of which is des-Ser1-cerebellin. Subcellular fractionation of cerebellum followed by ion-pairing HPLC analysis shows that both cerebellin and des-Ser1-cerebellin are enriched between 2.5- and 4-fold in the P2 crude synaptosomal fraction, suggesting their sequestration in some subcellular particle in vivo.

Keywords: endogenous peptide analysis, neural markers, synaptosomes

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Selected References

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