Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Nov;81(22):6963–6967. doi: 10.1073/pnas.81.22.6963

Isolation of brain fibroblast growth factor by heparin-Sepharose affinity chromatography: identity with pituitary fibroblast growth factor.

D Gospodarowicz, J Cheng, G M Lui, A Baird, P Böhlent
PMCID: PMC392056  PMID: 6594674

Abstract

Brain and pituitary fibroblast growth factors (FGF) have been purified to apparent homogeneity from crude tissue extracts by a three-step procedure, including salt precipitation, ion-exchange chromatography, and heparin-Sepharose affinity chromatography. Brain and pituitary FGF have similar amino acid compositions and are indistinguishable with respect to molecular weight (16,000 by polyacrylamide gel electrophoresis), retention behavior in reversed-phase high-performance liquid chromatography, and recognition by antibodies directed against the amino-terminal sequence of pituitary FGF. Brain FGF preparations purified by heparin-Sepharose contain, in addition to the major FGF molecular species, at least two additional forms of the growth factor, which appear to be very similar by all the above criteria, except for retention in high-performance liquid chromatography.

Full text

PDF
6963

Images in this article

6965Image
on p.6965
6966Image
on p.6966

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böhlen P., Baird A., Esch F., Ling N., Gospodarowicz D. Isolation and partial molecular characterization of pituitary fibroblast growth factor. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5364–5368. doi: 10.1073/pnas.81.17.5364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Böhlen P., Schroeder R. High-sensitivity amino acid analysis: methodology for the determination of amino acid compositions with less than 100 picomoles of peptides. Anal Biochem. 1982 Oct;126(1):144–152. doi: 10.1016/0003-2697(82)90120-8. [DOI] [PubMed] [Google Scholar]
  3. Gambarini A. G., Armelin H. A. Purification and partial characterization of an acidic fibroblast growth factor from bovine pituitary. J Biol Chem. 1982 Aug 25;257(16):9692–9697. [PubMed] [Google Scholar]
  4. Gospodarowicz D., Bialecki H., Greenburg G. Purification of the fibroblast growth factor activity from bovine brain. J Biol Chem. 1978 May 25;253(10):3736–3743. [PubMed] [Google Scholar]
  5. Gospodarowicz D., Bialecki H., Thakral T. K. The angiogenic activity of the fibroblast and epidermal growth factor. Exp Eye Res. 1979 May;28(5):501–514. doi: 10.1016/0014-4835(79)90038-1. [DOI] [PubMed] [Google Scholar]
  6. Gospodarowicz D., Greenburg G., Bialecki H., Zetter B. R. Factors involved in the modulation of cell proliferation in vivo and in vitro: the role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells. In Vitro. 1978 Jan;14(1):85–118. doi: 10.1007/BF02618177. [DOI] [PubMed] [Google Scholar]
  7. Gospodarowicz D. Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth. Nature. 1974 May 10;249(453):123–127. doi: 10.1038/249123a0. [DOI] [PubMed] [Google Scholar]
  8. Gospodarowicz D., Lui G. M., Cheng J. Purification in high yield of brain fibroblast growth factor by preparative isoelectric focusing at pH 9.6. J Biol Chem. 1982 Oct 25;257(20):12266–12276. [PubMed] [Google Scholar]
  9. Gospodarowicz D., Moran J., Braun D., Birdwell C. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4120–4124. doi: 10.1073/pnas.73.11.4120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gospodarowicz D. Purification of a fibroblast growth factor from bovine pituitary. J Biol Chem. 1975 Apr 10;250(7):2515–2520. [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Lemmon S. K., Riley M. C., Thomas K. A., Hoover G. A., Maciag T., Bradshaw R. A. Bovine fibroblast growth factor: comparison of brain and pituitary preparations. J Cell Biol. 1982 Oct;95(1):162–169. doi: 10.1083/jcb.95.1.162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Merril C. R., Goldman D., Sedman S. A., Ebert M. H. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science. 1981 Mar 27;211(4489):1437–1438. doi: 10.1126/science.6162199. [DOI] [PubMed] [Google Scholar]
  14. Shing Y., Folkman J., Sullivan R., Butterfield C., Murray J., Klagsbrun M. Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor. Science. 1984 Mar 23;223(4642):1296–1299. doi: 10.1126/science.6199844. [DOI] [PubMed] [Google Scholar]
  15. Tauber J. P., Cheng J., Massoglia S., Gospodarowicz D. High density lipoproteins and the growth of vascular endothelial cells in serum-free medium. In Vitro. 1981 Jun;17(6):519–530. doi: 10.1007/BF02633513. [DOI] [PubMed] [Google Scholar]
  16. Thomas K. A., Riley M. C., Lemmon S. K., Baglan N. C., Bradshaw R. A. Brain fibroblast growth factor: nonidentity with myelin basic protein fragments. J Biol Chem. 1980 Jun 25;255(12):5517–5520. [PubMed] [Google Scholar]
  17. Thomas K. A., Rios-Candelore M., Fitzpatrick S. Purification and characterization of acidic fibroblast growth factor from bovine brain. Proc Natl Acad Sci U S A. 1984 Jan;81(2):357–361. doi: 10.1073/pnas.81.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Westall F. C., Lennon V. A., Gospodarowicz D. Brain-derived fibroblast growth factor: identity with a fragment of the basic protein of myelin. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4675–4678. doi: 10.1073/pnas.75.10.4675. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES