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. 1984 Nov;81(22):7061–7065. doi: 10.1073/pnas.81.22.7061

Escherichia coli translational initiation factor IF3: a unique case of translational regulation.

L Gold, G Stormo, R Saunders
PMCID: PMC392076  PMID: 6390429

Abstract

The Escherichia coli translational initiation factor IF3 is encoded by an mRNA that has an unusual ribosome binding site. We have explored a mechanism that may account for the translation of IF3 and that provides regulation of the quantity of IF3 relative to ribosomes.

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Selected References

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  1. Brauer D., Wittmann-Liebold B. The primary structure of the initiation factor IF-3 from Escherichia coli. FEBS Lett. 1977 Jul 15;79(2):269–275. doi: 10.1016/0014-5793(77)80801-6. [DOI] [PubMed] [Google Scholar]
  2. Chaires J. B., Hawley D. A., Wahba A. J. Chain initiation factor 3 crosslinks to E. coli 30S and 50S ribosomal subunits and alters the UV absorbance spectrum of 70S ribosomes. Nucleic Acids Res. 1982 Sep 25;10(18):5681–5693. doi: 10.1093/nar/10.18.5681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ehresmann C., Ehresmann B., Millon R., Ebel J. P., Nurse K., Ofengand J. Cross-linking of the anticodon of Escherichia coli and Bacillus subtilis acetylvalyl-tRNA to the ribosomal P site. Characterization of a unique site in both E. coli 16S and yeast 18S ribosomal RNA. Biochemistry. 1984 Jan 31;23(3):429–437. doi: 10.1021/bi00298a006. [DOI] [PubMed] [Google Scholar]
  4. Gold L., Pribnow D., Schneider T., Shinedling S., Singer B. S., Stormo G. Translational initiation in prokaryotes. Annu Rev Microbiol. 1981;35:365–403. doi: 10.1146/annurev.mi.35.100181.002053. [DOI] [PubMed] [Google Scholar]
  5. Goss D. J., Parkhurst L. J., Wahba A. J. Kinetic studies on the interaction of chain initiation factor 3 with 70 S Escherichia coli ribosomes and subunits. J Biol Chem. 1982 Sep 10;257(17):10119–10127. [PubMed] [Google Scholar]
  6. Herr W., Chapman N. M., Noller H. F. Mechanism of ribosomal subunit association: discrimination of specific sites in 16 S RNA essential for association activity. J Mol Biol. 1979 Jun 5;130(4):433–449. doi: 10.1016/0022-2836(79)90433-9. [DOI] [PubMed] [Google Scholar]
  7. Howe J. G., Hershey J. W. Initiation factor and ribosome levels are coordinately controlled in Escherichia coli growing at different rates. J Biol Chem. 1983 Feb 10;258(3):1954–1959. [PubMed] [Google Scholar]
  8. Jay E., Seth A. K., Jay G. Specific binding of a chemically synthesized prokaryotic ribosome recognition site. Prospect for molecular cloning and expression of eukaryotic genes. J Biol Chem. 1980 May 10;255(9):3809–3812. [PubMed] [Google Scholar]
  9. Lestienne P., Dondon J., Plumbridge J. A., Howe J. G., Mayaux J. F., Springer M., Blanquet S., Hershey J. W., Grunberg-Manago M. Expression of the gene for Escherichia coli initiation factor IE-3 in vivo and in vitro. Eur J Biochem. 1982 Apr;123(3):483–488. doi: 10.1111/j.1432-1033.1982.tb06556.x. [DOI] [PubMed] [Google Scholar]
  10. Martin J., Webster R. E. The in vitro translation of a terminating signal by a single Escherichia coli ribosome. The fate of the subunits. J Biol Chem. 1975 Oct 25;250(20):8132–8139. [PubMed] [Google Scholar]
  11. Prince J. B., Taylor B. H., Thurlow D. L., Ofengand J., Zimmermann R. A. Covalent crosslinking of tRNA1Val to 16S RNA at the ribosomal P site: identification of crosslinked residues. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5450–5454. doi: 10.1073/pnas.79.18.5450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sacerdot C., Fayat G., Dessen P., Springer M., Plumbridge J. A., Grunberg-Manago M., Blanquet S. Sequence of a 1.26-kb DNA fragment containing the structural gene for E.coli initiation factor IF3: presence of an AUU initiator codon. EMBO J. 1982;1(3):311–315. doi: 10.1002/j.1460-2075.1982.tb01166.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stormo G. D., Schneider T. D., Gold L. M. Characterization of translational initiation sites in E. coli. Nucleic Acids Res. 1982 May 11;10(9):2971–2996. doi: 10.1093/nar/10.9.2971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stormo G. D., Schneider T. D., Gold L., Ehrenfeucht A. Use of the 'Perceptron' algorithm to distinguish translational initiation sites in E. coli. Nucleic Acids Res. 1982 May 11;10(9):2997–3011. doi: 10.1093/nar/10.9.2997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Suryanarayana T., Subramanian A. R. Separation of two forms of IF-3 in Escherichia coli by two-dimensional gel electrophoresis. FEBS Lett. 1977 Jul 15;79(2):264–268. doi: 10.1016/0014-5793(77)80800-4. [DOI] [PubMed] [Google Scholar]
  17. Thompson J. F., Hearst J. E. Structure of E. coli 16S RNA elucidated by psoralen crosslinking. Cell. 1983 Apr;32(4):1355–1365. doi: 10.1016/0092-8674(83)90316-1. [DOI] [PubMed] [Google Scholar]
  18. Van Der Laken K., Bakker-Steeneveld H., Van Knippenberg P. Polyuridylic acid-dependent binding of fMet-tRNA to Escherichia coli ribosomes and incorporation of formylmethionine into polyphenylalanine. FEBS Lett. 1979 Apr 15;100(2):230–234. doi: 10.1016/0014-5793(79)80340-3. [DOI] [PubMed] [Google Scholar]
  19. Vassilenko S. K., Carbon P., Ebel J. P., Ehresmann C. Topography of 16 S RNA in 30 S subunits and 70 S ribosomes accessibility to cobra venom ribonuclease. J Mol Biol. 1981 Nov 15;152(4):699–721. doi: 10.1016/0022-2836(81)90123-6. [DOI] [PubMed] [Google Scholar]
  20. Wagner R., Gassen H. G. Identification of a 16S rna sequence located in the decoding site of 30S ribosomes. FEBS Lett. 1976 Sep 1;67(3):312–315. doi: 10.1016/0014-5793(76)80554-6. [DOI] [PubMed] [Google Scholar]
  21. Wagner R., Gassen H. G. On the covalent binding of mRNA models to the part of the 16 S RNA which is located in the mRNA binding site of the 30 S ribosome. Biochem Biophys Res Commun. 1975 Jul 22;65(2):519–529. doi: 10.1016/s0006-291x(75)80178-1. [DOI] [PubMed] [Google Scholar]
  22. Woese C. R., Gutell R., Gupta R., Noller H. F. Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. Microbiol Rev. 1983 Dec;47(4):621–669. doi: 10.1128/mr.47.4.621-669.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wollenzien P., Hearst J. E., Thammana P., Cantor C. R. Base-pairing between distant regions of the Escherichia coli 16 S ribosomal RNA in solution. J Mol Biol. 1979 Nov 25;135(1):255–269. doi: 10.1016/0022-2836(79)90351-6. [DOI] [PubMed] [Google Scholar]
  24. van der Laken K., Bakker-Steeneveld H., Berkhout B., van Knippenberg P. H. The role of the codon and the initiation factor IF-2 in the selection of N-blocked aminoacyl-tRNA for initiation. Eur J Biochem. 1980 Feb;104(1):19–33. doi: 10.1111/j.1432-1033.1980.tb04394.x. [DOI] [PubMed] [Google Scholar]

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