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. 1984 Dec;81(23):7481–7484. doi: 10.1073/pnas.81.23.7481

Isolated uterine nuclei and cytosol receptors of aged rats exhibit impaired estrogenic stimulation of RNA polymerase II.

M Haji, R S Chuknyiska, G S Roth
PMCID: PMC392170  PMID: 6594698

Abstract

An in vitro cell-free system of uterine nuclei and cytosol receptors has been used to analyze the effects of aging on estrogen stimulation of RNA polymerase II activity. By using fixed concentrations of nuclei and cytoplasmic receptor--estrogen complexes (R-E2), it was found that mature nuclei are 3 times more efficient (155% vs. 57%) than old ones for stimulation of polymerase activity by mature R-E2. Meanwhile, mature R-E2 are 5 times more efficient (155% vs. 31%) than old ones in supporting such stimulation in mature nuclei. Stimulation by old cytosol R-E2 is so poor that it is essentially unaffected by nuclear age (31% with both mature and old nuclei). Finally, equimolar mixtures of mature and old cytosol R-E2 stimulate polymerase II activity in mature nuclei by 77%, a value intermediate between mature and old cytosols used separately. These results indicate that both nuclei and cytosol from old uteri are deficient in their ability to support estrogenic stimulation of RNA polymerase II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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