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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Dec;81(24):7985–7988. doi: 10.1073/pnas.81.24.7985

Intact proenkephalin is the major enkephalin-containing peptide produced in rat adrenal glands after denervation.

G Fleminger, R D Howells, D L Kilpatrick, S Udenfriend
PMCID: PMC392278  PMID: 6595672

Abstract

We have shown previously that enkephalin-containing peptides of high molecular weight increase 10- to 15-fold 3 days after adrenal denervation and have suggested that much of this material may represent newly synthesized proenkephalin. In this paper we report that most of the material appearing after denervation is indeed intact proenkephalin. The putative proenkephalin was partially purified by gel filtration chromatography and HPLC. A molecular size of about 25 kDa was estimated by gel filtration. On HPLC, the putative proenkephalin was eluted at a much higher propanol concentration than the 18.2-kDa enkephalin-containing peptide isolated previously. Treatment of this putative proenkephalin with endoproteinase Lys-C showed it contain [Met]-enkephalin, [Leu]enkephalin, [Met]enkephalin-Arg6-Phe7, and [Met]enkephalin-Arg6-Gly7-Leu8 in the same ratios as are found in proenkephalin as deduced from sequencing of proenkephalin cDNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böhlen P., Stein S., Dairman W., Udenfriend S. Fluorometric assay of proteins in the nanogram range. Arch Biochem Biophys. 1973 Mar;155(1):213–220. doi: 10.1016/s0003-9861(73)80023-2. [DOI] [PubMed] [Google Scholar]
  2. Comb M., Seeburg P. H., Adelman J., Eiden L., Herbert E. Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA. Nature. 1982 Feb 25;295(5851):663–666. doi: 10.1038/295663a0. [DOI] [PubMed] [Google Scholar]
  3. Fleminger G., Lahm H. W., Udenfriend S. Changes in rat adrenal catecholamines and proenkephalin metabolism after denervation. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3587–3590. doi: 10.1073/pnas.81.11.3587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gubler U., Seeburg P., Hoffman B. J., Gage L. P., Udenfriend S. Molecular cloning establishes proenkephalin as precursor of enkephalin-containing peptides. Nature. 1982 Jan 21;295(5846):206–208. doi: 10.1038/295206a0. [DOI] [PubMed] [Google Scholar]
  5. Howells R. D., Kilpatrick D. L., Bhatt R., Monahan J. J., Poonian M., Udenfriend S. Molecular cloning and sequence determination of rat preproenkephalin cDNA: sensitive probe for studying transcriptional changes in rat tissues. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7651–7655. doi: 10.1073/pnas.81.23.7651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kakidani H., Furutani Y., Takahashi H., Noda M., Morimoto Y., Hirose T., Asai M., Inayama S., Nakanishi S., Numa S. Cloning and sequence analysis of cDNA for porcine beta-neo-endorphin/dynorphin precursor. Nature. 1982 Jul 15;298(5871):245–249. doi: 10.1038/298245a0. [DOI] [PubMed] [Google Scholar]
  7. Kilpatrick D. L., Howells R. D., Fleminger G., Udenfriend S. Denervation of rat adrenal glands markedly increases preproenkephalin mRNA. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7221–7223. doi: 10.1073/pnas.81.22.7221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kimura S., Lewis R. V., Gerber L. D., Brink L., Rubinstein M., Stein S., Udenfriend S. Purification to homogeneity of camel pituitary pro-opiocortin, the common precursor of opioid peptides and corticotropin. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1756–1759. doi: 10.1073/pnas.76.4.1756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lahm H. W., Gerber L. D., Brink L., Kilpatrick D. L., Udenfriend S. Specific polyclonal antibodies to the carboxyl terminus of [Met]enkephalin-Arg6-Gly7-Leu8. Arch Biochem Biophys. 1983 Sep;225(2):422–429. doi: 10.1016/0003-9861(83)90049-8. [DOI] [PubMed] [Google Scholar]
  10. Lewis R. V., Stern A. S., Kilpatrick D. L., Gerber L. D., Rossier J., Stein S., Udenfriend S. Marked increases in large enkephalin-containing polypeptides in the rat adrenal gland following denervation. J Neurosci. 1981 Jan;1(1):80–82. doi: 10.1523/JNEUROSCI.01-01-00080.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lewis R. V., Stern A. S., Kimura S., Rossier J., Stein S., Udenfriend S. An about 50,000-dalton protein in adrenal medulla: a common precursor of [Met]- and [Leu]enkephalin. Science. 1980 Jun 27;208(4451):1459–1461. doi: 10.1126/science.7384787. [DOI] [PubMed] [Google Scholar]
  12. Mains R. E., Eipper B. A., Ling N. Common precursor to corticotropins and endorphins. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3014–3018. doi: 10.1073/pnas.74.7.3014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nakanishi S., Inoue A., Kita T., Nakamura M., Chang A. C., Cohen S. N., Numa S. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature. 1979 Mar 29;278(5703):423–427. doi: 10.1038/278423a0. [DOI] [PubMed] [Google Scholar]
  14. Noda M., Furutani Y., Takahashi H., Toyosato M., Hirose T., Inayama S., Nakanishi S., Numa S. Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin. Nature. 1982 Jan 21;295(5846):202–206. doi: 10.1038/295202a0. [DOI] [PubMed] [Google Scholar]
  15. Rubinstein M., Stein S., Udenfriend S. Characterization of pro-opiocortin, a precursor to opioid peptides and corticotropin. Proc Natl Acad Sci U S A. 1978 Feb;75(2):669–671. doi: 10.1073/pnas.75.2.669. [DOI] [PMC free article] [PubMed] [Google Scholar]

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