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. 1977 Feb;74(2):467–471. doi: 10.1073/pnas.74.2.467

Conditionally lethal ribosomal protein mutants: characterization of a locus required for modification of 50S subunit proteins.

S R Kushner, V F Maples, W S Champney
PMCID: PMC392310  PMID: 322127

Abstract

Mutagenized P1 bacteriophage were used to transduce a marker (aroE) adjacent to the cluster or ribosomal protein genes located at 72 min on the Escherichia coli chromosome. Linked temperature-sensitive transductants were isolated and characterized. A mutant unable to grow at 44 degrees was found to be defective in protein synthesis both in vivo and in vitro. At the restrictive temperature mutant cells lost all polyribosomes. Analysis of the ribosomal proteins revealed alterations in at least four 50S subunit proteins. The mutation (called rimE1, ribosomal protein modification) mapped between rpsE and aroE. It is suggested that the rimE locus is the structural gene for an activity that modifies a selected number of ribosomal proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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