Skip to main content
. 2014 Mar;20(3):421–427. doi: 10.1261/rna.042986.113

FIGURE 1.

FIGURE 1.

RNA tailing with biotin-ATP by S. pombe Cid1 and E. coli PAP. Two micromolar 5′ labeled RNA N22 was incubated with 0.5 mM ATP or N6-biotin-ATP as indicated and the following enzyme concentrations: 1 µM tCid1 (ATP reaction); 5 µM tCid1 (N6-biotin-ATP); 0.25 units/µL E. coli PAP (N6-biotin-ATP); and 0.025 units/µL E. coli PAP (ATP). After the incubation times indicated, aliquots were mixed with 8 M urea in 1× TBE and analyzed by denaturing polyacrylamide gel electrophoresis and phosphorimaging. DNA marker sizes are indicated.