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. 1977 Feb;74(2):520–523. doi: 10.1073/pnas.74.2.520

Virion-associated RNA polymerase required for bacteriophage N4 development.

S C Falco, K V Laan, L B Rothman-Denes
PMCID: PMC392321  PMID: 322130

Abstract

Escherichia coli phage N4 transcription is resistant to rifampicin, an inhibitor of the host RNA polymerase, even when the drug is added prior to infection. A rifampicin-resistant RNA polymerase has been detected in disrupted N4 virions. This enzyme shows a requirement for the four ribonucleoside 5'-triphosphates and exogenous denatured DNA. With denatured N4 DNA, the preferred template, transcription is asymmetric. The virion RNA polymerase apparently is necessary for phage development because a conditional lethal N4 mutant shows temperature-sensitive RNA synthesis in vivo as well as a temperature-sensitive RNA polymerase in disrupted virions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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