Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Feb;74(2):565–569. doi: 10.1073/pnas.74.2.565

Nerve growth factor receptors on human melanoma cells in culture.

R N Fabricant, J E De Larco, G J Todaro
PMCID: PMC392331  PMID: 265522

Abstract

Purified mouse nerve growth factor (NGF)radiolabeled with 125I was tested for its ability to bind to a variety of different cultured cells. NGF binds readily to human and hamster melanoma cells but does not bind to many other cell lines. The three cell lines with the highest number of NGF receptors were derived from metastatic melanomas. One of these lines, A875, was studied in detail and was shown to have approximately 7x10(5) NGF receptors per cell with an association constant of 1.0x10(9) liters/mole. The use of these cells in competition binding assays permits the detection of 0.25 ng of NGF in various biologic fluids. NGF can be shown to increase the survival, but not the division, of melanoma cells maintained in medium depleted of serum growth factors. This effect of NGF as a specific "survival factor" appears analogous to its effect on cultured sympathetic ganglion cells and on other cells derived from the neural crest.

Full text

PDF
565

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armelin H. A. Pituitary extracts and steroid hormones in the control of 3T3 cell growth. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2702–2706. doi: 10.1073/pnas.70.9.2702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Banerjee S. P., Snyder S. H., Cuatrecasas P., Greene L. A. Binding of nerve growth factor receptor in sympathetic ganglia. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2519–2523. doi: 10.1073/pnas.70.9.2519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COHEN S. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem. 1962 May;237:1555–1562. [PubMed] [Google Scholar]
  4. Cohen S., Carpenter G., Lembach K. J. Interaction of epidermal growth factor (EGF) with cultured fibroblasts. Adv Metab Disord. 1975;8:265–284. doi: 10.1016/b978-0-12-027308-9.50024-x. [DOI] [PubMed] [Google Scholar]
  5. DeLarco J., Todaro G. J. Membrane receptors for murine leukemia viruses: characterization using the purified viral envelope glycoprotein, gp71. Cell. 1976 Jul;8(3):365–371. doi: 10.1016/0092-8674(76)90148-3. [DOI] [PubMed] [Google Scholar]
  6. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Giard D. J., Aaronson S. A., Todaro G. J., Arnstein P., Kersey J. H., Dosik H., Parks W. P. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst. 1973 Nov;51(5):1417–1423. doi: 10.1093/jnci/51.5.1417. [DOI] [PubMed] [Google Scholar]
  8. Herrup K., Shooter E. M. Properties of the beta-nerve growth factor receptor in development. J Cell Biol. 1975 Oct;67(1):118–125. doi: 10.1083/jcb.67.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hollenberg M. D., Cuatrecasas P. Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2964–2968. doi: 10.1073/pnas.70.10.2964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LEVI-MONTALCINI R., MEYER H., HAMBURGER V. In vitro experiments on the effects of mouse sarcomas 180 and 37 on the spinal and sympathetic ganglia of the chick embryo. Cancer Res. 1954 Jan;14(1):49–57. [PubMed] [Google Scholar]
  11. Levi-Montalcini R., Angeletti P. U. Nerve growth factor. Physiol Rev. 1968 Jul;48(3):534–569. doi: 10.1152/physrev.1968.48.3.534. [DOI] [PubMed] [Google Scholar]
  12. MOORE G. E., LEHNER D. F., KIKUCHI Y., LESS L. A. Continuous culture of a melanotic cell line from the golden hamster. Science. 1962 Sep 21;137(3534):986–987. doi: 10.1126/science.137.3534.986. [DOI] [PubMed] [Google Scholar]
  13. Oger J., Arnason B. G., Pantazis N., Lehrich J., Young M. Synthesis of nerve growth factor by L and 3T3 cells in culture. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1554–1558. doi: 10.1073/pnas.71.4.1554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Revoltella R., Bertolini L., Pediconi M., Vigneti E. Specific binding of nerve growth factor (NGF) by murine C 1300 neuroblastoma cells. J Exp Med. 1974 Aug 1;140(2):437–451. doi: 10.1084/jem.140.2.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rudland P. S., Seifert W., Gospodarowicz D. Growth control in cultured mouse fibroblasts: induction of the pleiotypic and mitogenic responses by a purified growth factor. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2600–2604. doi: 10.1073/pnas.71.7.2600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Siggers D. C., Rogers J. G., Boyer S. H., Margolet L., Dorkin H., Banerjee S. P., Shooter E. M. Increased nerve-growth-factor beta-chain cross-reacting material in familial dysautonomia. N Engl J Med. 1976 Sep 16;295(12):629–634. doi: 10.1056/NEJM197609162951201. [DOI] [PubMed] [Google Scholar]
  17. Smith H. S., Scher C. D., Todaro G. J. Induction of cell division in medium lacking serum growth factor by SV40. Virology. 1971 May;44(2):359–370. doi: 10.1016/0042-6822(71)90267-4. [DOI] [PubMed] [Google Scholar]
  18. Todaro G. J., De Larco J. E., Cohen S. Transformation by murine and feline sarcoma viruses specifically blocks binding of epidermal growth factor to cells. Nature. 1976 Nov 4;264(5581):26–31. doi: 10.1038/264026a0. [DOI] [PubMed] [Google Scholar]
  19. Varon S., Nomura J., Shooter E. M. The isolation of the mouse nerve growth factor protein in a high molecular weight form. Biochemistry. 1967 Jul;6(7):2202–2209. doi: 10.1021/bi00859a043. [DOI] [PubMed] [Google Scholar]
  20. Young M., Oger J., Blanchard M. H., Asdourian H., Amos H., Arnason B. G. Secretion of a nerve growth factor by primary chick fibroblast cultures. Science. 1975 Jan 31;187(4174):361–362. doi: 10.1126/science.1167427. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES