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. 1977 Feb;74(2):653–656. doi: 10.1073/pnas.74.2.653

Appearance of a chromatin protein during the erythroid differentiation of Friend virus-transformed cells.

F Keppel, B Allet, H Eisen
PMCID: PMC392350  PMID: 265529

Abstract

Chromatin proteins from erythroleukemia cells transformed by Friend virus were analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. A new chromosomal protein, apparent molecular weight 25,000, was shown to appear during erythroid differentiation of the cells induced by dimethyl sulfoxide or hexamethylenebisacetamide. This protein is tightly bound to the chromatin and does not appear to be related to any of the known histones. It is also demonstrated that this protein is not induced in a dimethyl sulfoxide-resistant variant of these cells.

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Selected References

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  1. Allet B., Katagiri K. J., Gesteland R. F. Characterization of polypeptides made in vitro from bacteriophage lambda DNA. J Mol Biol. 1973 Aug 25;78(4):589–600. doi: 10.1016/0022-2836(73)90281-7. [DOI] [PubMed] [Google Scholar]
  2. Arnold E. A., Buksas M. M., Young K. E. A comparative study of some properties of chromatin from two "minimal deviation" hepatomas. Cancer Res. 1973 Jun;33(6):1169–1176. [PubMed] [Google Scholar]
  3. Bhorjee J. S., Pederson T. Nonhistone chromosomal proteins in synchronized HeLa cells. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3345–3349. doi: 10.1073/pnas.69.11.3345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DULBECCO R., FREEMAN G. Plaque production by the polyoma virus. Virology. 1959 Jul;8(3):396–397. doi: 10.1016/0042-6822(59)90043-1. [DOI] [PubMed] [Google Scholar]
  5. Elgin S. C., Weintraub H. Chromosomal proteins and chromatin structure. Annu Rev Biochem. 1975;44:725–774. doi: 10.1146/annurev.bi.44.070175.003453. [DOI] [PubMed] [Google Scholar]
  6. Friend C., Patuleia M. C., De Harven E. Erythrocytic maturation in vitro of murine (Friend) virus-induced leukemic cells. Natl Cancer Inst Monogr. 1966 Sep;22:505–522. [PubMed] [Google Scholar]
  7. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gaedicke G., Abedin Z., Dube S. K., Kluge N., Neth R., Steinheider G., Weimann B. J., Ostertag W. Control of globin synthesis during DMSO-induced differentiation of mouse erythroleukemic cells in culture. Hamatol Bluttransfus. 1974;14:278–287. [PubMed] [Google Scholar]
  9. Garel A., Mazen A., Champagne M., Sautiere P., Kmiecik D., Loy O., Biserte G. Chicken erythrocyte histone H5; I. Amino terminal sequence (70 residues). FEBS Lett. 1975 Feb 1;50(2):195–199. doi: 10.1016/0014-5793(75)80487-x. [DOI] [PubMed] [Google Scholar]
  10. Gusella J. F., Housman D. Induction of erythroid differentiation in vitro by purines and purine analogues. Cell. 1976 Jun;8(2):263–269. doi: 10.1016/0092-8674(76)90010-6. [DOI] [PubMed] [Google Scholar]
  11. Ikawa Y., Furusawa M., Sugano H. Erythrocyte membrane-specific antigens in Friend virus-induced leukemia cells. Bibl Haematol. 1973;39:955–967. doi: 10.1159/000427928. [DOI] [PubMed] [Google Scholar]
  12. Kabat D., Sherton C. C., Evans L. H., Bigley R., Koler R. D. Synthesis of erythrocyte-specific proteins in cultured friend leukemia cells. Cell. 1975 Jul;5(3):331–338. doi: 10.1016/0092-8674(75)90109-9. [DOI] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Leder A., Leder P. Butyric acid, a potent inducer of erythroid differentiation in cultured erythroleukemic cells. Cell. 1975 Jul;5(3):319–322. doi: 10.1016/0092-8674(75)90107-5. [DOI] [PubMed] [Google Scholar]
  15. McClintock P. R., Papaconstantinou J. Regulation of hemoglobin synthesis in a murine erythroblastic leukemic cell: the requirement for replication to induce hemoglobin synthesis. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4551–4555. doi: 10.1073/pnas.71.11.4551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. NEELIN J. M., CALLAHAN P. X., LAMB D. C., MURRAY K. THE HISTONES OF CHICKEN ERYTHROCYTE NUCLEI. Can J Biochem. 1964 Dec;42:1743–1752. doi: 10.1139/o64-185. [DOI] [PubMed] [Google Scholar]
  17. Newman S. A., Birnbaum J., Yeoh G. C. Loss of a non-histone chromatin protein parallels in vitro differentiation of cartilage. Nature. 1976 Feb 5;259(5542):417–418. doi: 10.1038/259417a0. [DOI] [PubMed] [Google Scholar]
  18. Ostertag W., Melderis H., Steinheider G., Kluge N., Dube S. Synthesis of mouse haemoglobin and globin mRNA in leukaemic cell cultures. Nat New Biol. 1972 Oct 25;239(95):231–234. doi: 10.1038/newbio239231a0. [DOI] [PubMed] [Google Scholar]
  19. Panyim S., Chalkley R. A new histone found only in mammalian tissues with little cell division. Biochem Biophys Res Commun. 1969 Dec 4;37(6):1042–1049. doi: 10.1016/0006-291x(69)90237-x. [DOI] [PubMed] [Google Scholar]
  20. Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
  21. Peterson J. L., McConkey E. H. Proteins of Friend leukemia cells. Comparison of hemoglobin-synthesizing and noninduced populations. J Biol Chem. 1976 Jan 25;251(2):555–558. [PubMed] [Google Scholar]
  22. Reuben R. C., Wife R. L., Breslow R., Rifkind R. A., Marks P. A. A new group of potent inducers of differentiation in murine erythroleukemia cells. Proc Natl Acad Sci U S A. 1976 Mar;73(3):862–866. doi: 10.1073/pnas.73.3.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ross J., Ikawa Y., Leder P. Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3620–3623. doi: 10.1073/pnas.69.12.3620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sherton C. C., Kabat D. Changes in RNA and protein metabolism preceding onset of hemoglobin synthesis in cultured Friend leukemia cells. Dev Biol. 1976 Jan;48(1):118–131. doi: 10.1016/0012-1606(76)90051-8. [DOI] [PubMed] [Google Scholar]
  25. Studier F. W. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. doi: 10.1126/science.176.4033.367. [DOI] [PubMed] [Google Scholar]
  26. Tanaka M., Levy J., Terada M., Breslow R., Rifkind R. A., Marks P. A. Induction of erythroid differentiation in murine virus infected eythroleukemia cells by highly polar compounds. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1003–1006. doi: 10.1073/pnas.72.3.1003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wu F. C., Elgin S. C., Hood L. E. Nonhistone chromosomal proteins of rat tissues. A comparative study by gel electrophoresis. Biochemistry. 1973 Jul 17;12(15):2792–2797. doi: 10.1021/bi00739a003. [DOI] [PubMed] [Google Scholar]

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