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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Feb;74(2):676–678. doi: 10.1073/pnas.74.2.676

Lymphocyte mitogenesis induced by a mammalian liver protein that specifically binds desialylated glycoproteins.

A Novogrodsky, G Ashwell
PMCID: PMC392355  PMID: 265530

Abstract

A purified rabbit liver membrane protein that binds desialylated glycoproteins has been shown to be a mitogen for human peripheral lymphocytes. The mitogenic activity is specific for desialylated thymus-derived (T)-cells. The loss of mitogenicity upon exposure of the binding protein to neuraminidase and the inhibitory potency of asialo-orosomucoid support the conclusion that the site involved in the binding of asialoglycoproteins is also responsible for the mitogenic effect on desialylated lymphocytes. The potential relevance of this phenomenon to the etiology of hepatocellular necrosis is considered.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashwell G., Morell A. G. The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins. Adv Enzymol Relat Areas Mol Biol. 1974;41(0):99–128. doi: 10.1002/9780470122860.ch3. [DOI] [PubMed] [Google Scholar]
  2. Gordon Julius A., Blumberg Shmaryahu, Lis Halina, Sharon Nathan. Purification of soybean agglutinin by affinity chromatography On sepharose-N-epsilon-aminocaproyl-beta-D-galactopyranosylamine. FEBS Lett. 1972 Aug 1;24(2):193–196. doi: 10.1016/0014-5793(72)80765-8. [DOI] [PubMed] [Google Scholar]
  3. Hartzman R. J., Segall M., Bach M. L., Bach F. H. Histocompatibility matching. VI. Miniaturization of the mixed leukocyte culture test: a preliminary report. Transplantation. 1971 Mar;11(3):268–273. doi: 10.1097/00007890-197103000-00005. [DOI] [PubMed] [Google Scholar]
  4. Hudgin R. L., Pricer W. E., Jr, Ashwell G., Stockert R. J., Morell A. G. The isolation and properties of a rabbit liver binding protein specific for asialoglycoproteins. J Biol Chem. 1974 Sep 10;249(17):5536–5543. [PubMed] [Google Scholar]
  5. Kawasaki T., Ashwell G. Chemical and physical properties of an hepatic membrane protein that specifically binds asialoglycoproteins. J Biol Chem. 1976 Mar 10;251(5):1296–1302. [PubMed] [Google Scholar]
  6. Lis H., Sharon N. The biochemistry of plant lectins (phytohemagglutinins). Annu Rev Biochem. 1973;42(0):541–574. doi: 10.1146/annurev.bi.42.070173.002545. [DOI] [PubMed] [Google Scholar]
  7. Lohrmann H. P., Novikovs L., Graw R. G., Jr Cellular interactions in the proliferative response of human T and B lymphocytes to phytomitogens and allogeneic lymphocytes. J Exp Med. 1974 Jun 1;139(6):1553–1567. doi: 10.1084/jem.139.6.1553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lotan R., Skutelsky E., Danon D., Sharon N. The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea). J Biol Chem. 1975 Nov 10;250(21):8518–8523. [PubMed] [Google Scholar]
  9. Novogrodsky A., Katchalski E. Transformation of neuraminidase-treated lymphocytes by soybean agglutinin. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2515–2518. doi: 10.1073/pnas.70.9.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Novogrodsky A., Lotan R., Ravid A., Sharon N. Peanut agglutinin, a new mitogen that binds to galactosyl sites exposed after neuraminidase treatment. J Immunol. 1975 Nov;115(5):1243–1248. [PubMed] [Google Scholar]
  11. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  12. Pricer W. E., Jr, Ashwell G. The binding of desialylated glycoproteins by plasma membranes of rat liver. J Biol Chem. 1971 Aug 10;246(15):4825–4833. [PubMed] [Google Scholar]
  13. Stockert R. J., Morell A. G., Scheinberg I. H. Mammalian hepatic lectin. Science. 1974 Oct 25;186(4161):365–366. doi: 10.1126/science.186.4161.365. [DOI] [PubMed] [Google Scholar]
  14. Van Lenten L., Ashwell G. Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins. J Biol Chem. 1971 Mar 25;246(6):1889–1894. [PubMed] [Google Scholar]
  15. Woodruff J. J., Gesner B. M. The effect of neuraminidase on the fate of transfused lymphocytes. J Exp Med. 1969 Mar 1;129(3):551–567. doi: 10.1084/jem.129.3.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Woodruff J. J., Woodruff J. F. Influenza A virus interaction with murine lymphocytes. I. The influence of influenza virus A/Japan 305 (H2N2) on the pattern of migration of recirculating lymphocytes. J Immunol. 1976 Sep;117(3):852–858. [PubMed] [Google Scholar]
  17. Woodruff J. J., Woodruff J. F. Influenza A virus interaction with murine lymphocytes. II. Changes in lymphocyte surface properties induced by influenza virus A/Japan 305 (H2N2). J Immunol. 1976 Sep;117(3):859–864. [PubMed] [Google Scholar]

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