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. 1978 Apr;75(4):1647–1651. doi: 10.1073/pnas.75.4.1647

Species-specific cellular DNA-binding proteins expressed in mouse cells transformed by chemical carcinogens.

A Sen, G J Todaro
PMCID: PMC392395  PMID: 205864

Abstract

Mouse cells transformed by DNA and RNA tumor viruses and by chemical carcinogens have been examined for the presence of specific DNA-binding proteins by DNA-cellulose chromatography. Using mouse DNA-cellulose we have obtained single-stranded DNA-binding proteins from two clones transformed by chemical carcinogens. Simian virus 40 transformants also have a DNA-binding protein [the tumor (T) antigen] that binds to mouse and human DNA with comparable affinity. Mouse sarcoma virus-transformed cells and two other chemically transformed clones showed no difference in DNA-binding protein pattern compared to the untransformed parental cell. The DNA-binding proteins isolated from the chemically transformed cell clones are between 25,000 and 30,000 daltons by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. These cellular "T proteins" bind to the homologous mouse cellular DNA with a higher affinity than to heterologous human cellular DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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