Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 May;75(5):2085–2087. doi: 10.1073/pnas.75.5.2085

Hydrolysis of poly (A) to adenine nucleotides by purified poly (A) polymerase.

A K Abraham, S T Jacob
PMCID: PMC392495  PMID: 27783

Abstract

Highly purified poly(A) polymerase (polynucleotide adenylyltransferase, EC 2.7.7.19), which synthesizes poly(A) from ATP substrate, can also catalyze hydrolysis of poly(A). The enzyme, designated as poly(A) hydrolase, requires either Mn2+ or Mg2+ for activity. Although AMP is the predominant product of the reaction, ADP and ATP are also formed. The enzyme is a 3'-exonuclease that does not degrade poly(A) associated with poly(A) poly(U) helical structure. AMP, ADP, and ATP inhibit the hydrolytic reaction. These data suggest that (i) the levels of adenine nucleotides regulate synthesis and degradation of poly(A), (ii) poly(A) itself is a storage form of adenine nucleotides, (iii) the hydrolytic reaction is responsible for poly(A) shortening or turnover observed in vivo, and (iv) the synthetic and hydrolytic activities are functions of the same protein molecule.

Full text

PDF
2085

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cameron V., Uhlenbeck O. C. 3'-Phosphatase activity in T4 polynucleotide kinase. Biochemistry. 1977 Nov 15;16(23):5120–5126. doi: 10.1021/bi00642a027. [DOI] [PubMed] [Google Scholar]
  2. Edmonds M., Winters M. A. Polyadenylate polymerases. Prog Nucleic Acid Res Mol Biol. 1976;17:149–179. doi: 10.1016/s0079-6603(08)60069-0. [DOI] [PubMed] [Google Scholar]
  3. Gallwitz D., Traub U., Traub P. Fate of histone messenger RNA in mengovirus-infected Ehrlich ascites tumor cells. Eur J Biochem. 1977 Dec 1;81(2):387–393. doi: 10.1111/j.1432-1033.1977.tb11963.x. [DOI] [PubMed] [Google Scholar]
  4. Greenberg J. R., Perry R. P. The isolation and characterization of steady-state labeled messenger RNA from L-cells. Biochim Biophys Acta. 1972 Dec 6;287(2):361–366. doi: 10.1016/0005-2787(72)90386-3. [DOI] [PubMed] [Google Scholar]
  5. Jacob S. T., Schindler D. G. Polyriboadenylate polymerase solubilized from rat liver mitochondria. Biochem Biophys Res Commun. 1972 Jul 11;48(1):126–134. doi: 10.1016/0006-291x(72)90353-1. [DOI] [PubMed] [Google Scholar]
  6. Lamed R., Levin Y., Wilchek M. Covalent coupling of nucleotides to agarose for affinity chromatography. Biochim Biophys Acta. 1973 Apr 28;304(2):231–235. doi: 10.1016/0304-4165(73)90239-0. [DOI] [PubMed] [Google Scholar]
  7. Lazarus H. M., Sporin M. B. Purification and properties of a nuclear exoribonuclease from Ehrlich ascites tumor cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1386–1393. doi: 10.1073/pnas.57.5.1386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mendecki J., Lee S. Y., Brawerman G. Characteristics of the polyadenylic acid segment associated with messenger ribonucleic acid in mouse sarcoma 180 ascites cells. Biochemistry. 1972 Feb 29;11(5):792–798. doi: 10.1021/bi00755a018. [DOI] [PubMed] [Google Scholar]
  9. RAZZELL W. E., KHORANA H. G. Studies on polynucleotides. IV. Enzymic degradation; the stepwise action of venom phosphodiesterase on deoxyribo-oligonucleotides. J Biol Chem. 1959 Aug;234(8):2114–2117. [PubMed] [Google Scholar]
  10. Rose K. M., Jacob S. T. Inhibition of the polyadenylation reaction in vitro by polyamines. Arch Biochem Biophys. 1976 Aug;175(2):748–753. doi: 10.1016/0003-9861(76)90568-3. [DOI] [PubMed] [Google Scholar]
  11. Rose K. M., Jacob S. T. Nuclear poly(A) polymerase from rat liver and a hepatoma. Comparison of properties, molecular weights and amino acid compositions. Eur J Biochem. 1976 Aug 1;67(1):11–21. doi: 10.1111/j.1432-1033.1976.tb10626.x. [DOI] [PubMed] [Google Scholar]
  12. Rose K. M., Jacob S. T. Poly(adenylic acid) synthesis in isolated rat liver mitochondria. Biochemistry. 1976 Nov 16;15(23):5046–5052. doi: 10.1021/bi00668a015. [DOI] [PubMed] [Google Scholar]
  13. Rose K. M., Morris H. P., Jacob S. T. Mitochondrial poly(A) polymerase from a poorly differentiated hepatoma: purification and characteristics. Biochemistry. 1975 Mar 11;14(5):1025–1032. doi: 10.1021/bi00676a022. [DOI] [PubMed] [Google Scholar]
  14. Tsiapalis C. M., Dorson J. W., Bollum F. J. Purification of terminal riboadenylate transferase from calf thymus gland. J Biol Chem. 1975 Jun 25;250(12):4486–4496. [PubMed] [Google Scholar]
  15. Wilkie N. M., Smellie R. M. Chain extension of ribonucleic acid by enzymes from rat liver cytoplasm. Biochem J. 1968 Oct;109(4):485–494. doi: 10.1042/bj1090485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Winters M. A., Edmonds M. A poly(A) polymerase from calf thymus. Characterization of the reaction product and the primer requirement. J Biol Chem. 1973 Jul 10;248(13):4763–4768. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES