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. 1978 May;75(5):2165–2169. doi: 10.1073/pnas.75.5.2165

Large and small tumor antigens from simian virus 40 have identical amino termini mapping at 0.65 map units.

E Paucha, A Mellor, R Harvey, A E Smith, R M Hewick, M D Waterfield
PMCID: PMC392512  PMID: 209456

Abstract

Large and small tumor (T)antigens of simian virus 40 were synthesized in vitro with L-cell extracts that had been treated by the method of Palmiter to prevent amino-terminal acetylation of nascent proteins. Partial amino-terminal amino acid sequences of both forms of T-antigen were determined and found to be identical. Methionine residues were located at positions 1 and 14, a lysine residue at position 3, and leucine residues at positions 5, 11, 13,16, 17, and 19. These amino acid sequence data match perfectly the amino acid sequence predicted from a sequence of nucleotides in the E strand of simian virus 40 DNA which begins near the junction between HindII/III fragments A and C at about 0.65 map units. This strongly suggests that the sequence coding for the amino terminus of both proteins is located at this position. Furthermore, the data are consistent with a model for the synthesis of both forms of T-antigen that predicts that (i) small T-antigen is coded for by a sequence of nucleotides from the 5' end of the early region and (ii) large T-antigen is coded for by nucleotide sequences from two noncontiguous regions of simian virus 40 DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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