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. 1978 May;75(5):2311–2314. doi: 10.1073/pnas.75.5.2311

Interactions between adenovirus, a tumor promoter, and chemical carcinogens in transformation of rat embryo cell cultures.

P B Fisher, I B Weinstein, D Eisenberg, H S Ginsberg
PMCID: PMC392542  PMID: 276873

Abstract

The tumor-promoting agent 12-O-tetradecanoyl-phorbol-13-acetate (TPA) caused a 2- to 3-fold enhancement of transformation of secondary rat embryo cells that had been injected with a temperature-sensitive mutant of adenovirus type 5(H5ts 125). In addition, transformed foci appeared earlier and were larger in cultures grown in the presence of TPA. The addition of TPA could be delayed until up to 7 days after viral injection and still enhancement was observed. Exposure of the cells to 7,12-dimethylbenz[a]pyrene prior to H5ts125 infection also resulted in a 2- to 4-fold enhancement of transformation, and this enhancement was further augmented 2- to 3-fold when cells were grown in TPA after virus infection. Whereas TPA did not enhance the cloning efficiency of normal rat embryo cells, it did enhance the cloning efficiency of isolated colonies of adenovirus-transformed cells when these were grown alone or cocultured with a 100-fold excess of normal rat embryo cells. The enhancement of adenovirus transformation by TPA appears to be due to its ability to facilitate expression of the transformed state rather than an effect on virus uptake or integration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boutwell R. K. The function and mechanism of promoters of carcinogenesis. CRC Crit Rev Toxicol. 1974 Jan;2(4):419–443. doi: 10.3109/10408447309025704. [DOI] [PubMed] [Google Scholar]
  2. Casto B. C. Adenovirus transformation of hamster embryo cells. J Virol. 1968 Apr;2(4):376–383. doi: 10.1128/jvi.2.4.376-383.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Casto B. C., Pieczynski W. J., DiPaolo J. A. Enhancement of adenovirus transformation by pretreatment of hamster cells with carcinogenic polycyclic hydrocarbons. Cancer Res. 1973 Apr;33(4):819–824. [PubMed] [Google Scholar]
  4. Coggin J. H., Jr Enhanced virus transformation of hamster embryo cells in vitro. J Virol. 1969 May;3(5):458–462. doi: 10.1128/jvi.3.5.458-462.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doerfler W. Integration of the deoxyribonucleic acid of adenovirus type 12 into the deoxyribonucleic acid of baby hamster kidney cells. J Virol. 1970 Nov;6(5):652–666. doi: 10.1128/jvi.6.5.652-666.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ensinger M. J., Ginsberg H. S. Selection and preliminary characterization of temperature-sensitive mutants of type 5 adenovirus. J Virol. 1972 Sep;10(3):328–339. doi: 10.1128/jvi.10.3.328-339.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Freeman A. E., Black P. H., Vanderpool E. A., Henry P. H., Austin J. B., Huebner R. J. Transformation of primary rat embryo cells by adenovirus type 2. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1205–1212. doi: 10.1073/pnas.58.3.1205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Freeman A. E., Black P. H., Wolford R., Huebner R. J. Adenovirus type 12-rat embryo transformation system. J Virol. 1967 Apr;1(2):362–367. doi: 10.1128/jvi.1.2.362-367.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Freeman A. E., Price P. J., Igel H. J., Young J. C., Maryak J. M., Huebner R. J. Morphological transformation of rat embryo cells induced by diethylnitrosamine and murine leukemia viruses. J Natl Cancer Inst. 1970 Jan;44(1):65–78. [PubMed] [Google Scholar]
  10. Ginsberg H. S., Ensinger M. J., Kauffman R. S., Mayer A. J., Lundholm U. Cell transformation: a study of regulation with types 5 and 12 adenovirus temperature-sensitive mutants. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):419–426. doi: 10.1101/sqb.1974.039.01.054. [DOI] [PubMed] [Google Scholar]
  11. Hirai K., Defendi V., Diamond L. Enhancement of simian virus 40 transformation and integration by 4-nitroquinoline 1-oxide. Cancer Res. 1974 Dec;34(12):3497–3500. [PubMed] [Google Scholar]
  12. Lasne C., Gentil A., Chouroulinkov I. Two-stage malignant transformation of rat fibroblasts in tissue culture. Nature. 1974 Feb 15;247(5441):490–491. doi: 10.1038/247490a0. [DOI] [PubMed] [Google Scholar]
  13. Ledinko N., Evans M. Enhancement of adenovirus transformation of hamster cells by N-methyl-N'-nitro-N-nitrosoguanidine, caffeine, and hydroxylamine. Cancer Res. 1973 Nov;33(11):2936–2938. [PubMed] [Google Scholar]
  14. Mayer A. J., Ginsberg H. S. Persistence of type 5 adenovirus DNA in cells transformed by temperature-sensitive mutant, H5ts125. Proc Natl Acad Sci U S A. 1977 Feb;74(2):785–788. doi: 10.1073/pnas.74.2.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mondal S., Brankow D. W., Heidelberger C. Two-stage chemical oncogenesis in cultures of C3H/10T1/2 cells. Cancer Res. 1976 Jul;36(7 Pt 1):2254–2260. [PubMed] [Google Scholar]
  16. Mondal S., Heidelberger C. Transformation of C3H/10T1/2CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature. 1976 Apr 22;260(5553):710–711. doi: 10.1038/260710a0. [DOI] [PubMed] [Google Scholar]
  17. Sivak A., Van Duuren B. L. Phenotypic expression of transformation: induction in cell culture by a phorbol ester. Science. 1967 Sep 22;157(3795):1443–1444. doi: 10.1126/science.157.3795.1443. [DOI] [PubMed] [Google Scholar]
  18. Van Duuren B. L. Tumor-promoting agents in two-stage carcinogenesis. Prog Exp Tumor Res. 1969;11:31–68. doi: 10.1159/000391388. [DOI] [PubMed] [Google Scholar]
  19. Wigler M., Weinstein I. B. Tumour promotor induces plasminogen activator. Nature. 1976 Jan 22;259(5540):232–233. doi: 10.1038/259232a0. [DOI] [PubMed] [Google Scholar]

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