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Association reaction kinetics for rFVIIa binding to RL platelets. RL platelets (at 9.0 × 105/ul) were mixed with rFVIIa (at 10 uM) in citrated saline with calcium, then portions were withdrawn as a function of time and analyzed with Western Analysis as detailed in the Materials and methods section. Inset: Western analysis with and without added calcium in the reaction mixture.
