Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 May;75(5):2396–2400. doi: 10.1073/pnas.75.5.2396

Natural species-restricted attachment of human and murine T lymphocytes to various cells.

U Galili, N Galili, F Vánky, E Klein
PMCID: PMC392560  PMID: 307762

Abstract

Murine and human T lymphocytes bear on their surface a receptor that confers on them the ability to attach to a variety of target cells from the same species, derived in vivo and in vitro. Thymocytes and activated T cells attached readily to target cells, while blood T lymphocytes were able to do so only after the removal of sialic acid from either their cell membrane or that of the target cell. The natural attachment (NA) receptor and the corresponding site on the target cells are trypsin sensitive and the conjugation between them is temperature dependent. The phenomenon may be a manifestation of self recognition in a broader sense--recognizing the species--which is also reflected in the reactivity of mitogen-activated T cells and specific immune responses against allo- or other antigens expressed on target cell surfaces.

Full text

PDF
2396

Images in this article

2397Image
on p.2397

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  2. Coffino P., Laskov R., Scharff M. D. Immunoglobulin production: method for quantitatively detecting variant myeloma cells. Science. 1970 Jan 9;167(3915):186–188. doi: 10.1126/science.167.3915.186. [DOI] [PubMed] [Google Scholar]
  3. FOLEY G. E., LAZARUS H., FARBER S., UZMAN B. G., BOONE B. A., MCCARTHY R. E. CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA. Cancer. 1965 Apr;18:522–529. doi: 10.1002/1097-0142(196504)18:4<522::aid-cncr2820180418>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  4. GORER P. A. Studies in antibody response of mice to tumour inoculation. Br J Cancer. 1950 Dec;4(4):372–379. doi: 10.1038/bjc.1950.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Galili U., Caine M., Servadio C., Schlesinger M. Specific attachment of T-lymphocytes from cancer patients to tumor cells. Cancer Lett. 1977 Sep;3(3-4):121–124. doi: 10.1016/s0304-3835(77)94882-0. [DOI] [PubMed] [Google Scholar]
  6. Galili U., Klein E., Schlesinger M. Human T lymphocyte receptors for sheep red blood cells and specific antigens: are they identical sites on the cell membrane? J Immunol. 1977 Jul;119(1):104–109. [PubMed] [Google Scholar]
  7. Galili U., Schlesinger M. Studies on the formation of E-rosettes by human T lymphocytes and thymus cells. Effects of temperature, metabolic inhibitors and anti-T sera. Isr J Med Sci. 1975 Dec;11(12):1357–1367. [PubMed] [Google Scholar]
  8. Galili U., Schlesinger M. Subpopulations of human thymus cells differing in their capacity to form stable E-rosettes and in their immunologic reactivity. J Immunol. 1975 Sep;115(3):827–833. [PubMed] [Google Scholar]
  9. Galili U., Schlesinger M. The formation of stable E-rosettes by human T lymphocytes activated in mixed lymphocyte reactions. J Immunol. 1976 Sep;117(3):730–735. [PubMed] [Google Scholar]
  10. Glynn J. P., McCoy J. L., Fefer A. Cross-resistance to the transplantation of syngeneic Friend, Moloney, and Rauscher virus-induced tumors. Cancer Res. 1968 Mar;28(3):434–439. [PubMed] [Google Scholar]
  11. Hinuma Y., Grace J. T., Jr Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long-term cultures. Proc Soc Exp Biol Med. 1967 Jan;124(1):107–111. doi: 10.3181/00379727-124-31677. [DOI] [PubMed] [Google Scholar]
  12. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 1968 Jul;28(7):1300–1310. [PubMed] [Google Scholar]
  13. Klein G., Dombos L., Gothoskar B. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int J Cancer. 1972 Jul 15;10(1):44–57. doi: 10.1002/ijc.2910100108. [DOI] [PubMed] [Google Scholar]
  14. Klein G., Giovanella B., Westman A., Stehlin J. S., Mumford D. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology. 1975;5(6):319–334. doi: 10.1159/000149930. [DOI] [PubMed] [Google Scholar]
  15. Klein G., Klein E., Haughton G. Variation of antigenic characteristics between different mouse lymphomas induced by the Moloney virus. J Natl Cancer Inst. 1966 Apr;36(4):607–621. doi: 10.1093/jnci/36.4.607. [DOI] [PubMed] [Google Scholar]
  16. Lieberman I., Ove P. ISOLATION AND STUDY OF MUTANTS FROM MAMMALIAN CELLS IN CULTURE. Proc Natl Acad Sci U S A. 1959 Jun;45(6):867–872. doi: 10.1073/pnas.45.6.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lozzio C. B., Lozzio B. B. Cytotoxicity of a factor isolated from human spleen. J Natl Cancer Inst. 1973 Feb;50(2):535–538. doi: 10.1093/jnci/50.2.535. [DOI] [PubMed] [Google Scholar]
  18. MORRIS N. R., FISCHER G. A. Studies concerning inhibition of the synthesis of deoxycytidine by phosphorylated derivatives of thymidine. Biochim Biophys Acta. 1960 Jul 29;42:183–184. doi: 10.1016/0006-3002(60)90777-0. [DOI] [PubMed] [Google Scholar]
  19. Martz E. Early steps in specific tumor cell lysis by sensitized mouse T lymphocytes. I. Resolution and characterization. J Immunol. 1975 Jul;115(1):261–267. [PubMed] [Google Scholar]
  20. Menezes J., Leibold W., Klein G., Clements G. Establishment and characterization of an Epstein-Barr virus (EBC)-negative lymphoblastoid B cell line (BJA-B) from an exceptional, EBV-genome-negative African Burkitt's lymphoma. Biomedicine. 1975 Jul;22(4):276–284. [PubMed] [Google Scholar]
  21. Minowada J., Onuma T., Moore G. E. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst. 1972 Sep;49(3):891–895. [PubMed] [Google Scholar]
  22. Mohit B., Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science. 1971 Jan 8;171(3966):75–77. doi: 10.1126/science.171.3966.75. [DOI] [PubMed] [Google Scholar]
  23. Nordling S., Andersson L. C., Häyry P. Thymus-dependent and thymus-independent lymphocyte separation: relation to exposed sialic acid on cell surface. Science. 1972 Dec 1;178(4064):1001–1002. doi: 10.1126/science.178.4064.1001. [DOI] [PubMed] [Google Scholar]
  24. PULVERTAFT J. V. A STUDY OF MALIGNANT TUMOURS IN NIGERIA BY SHORT-TERM TISSUE CULTURE. J Clin Pathol. 1965 May;18:261–273. doi: 10.1136/jcp.18.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. SIMONSEN M. Graft versus host reactions. Their natural history, and applicability as tools of research. Prog Allergy. 1962;6:349–467. [PubMed] [Google Scholar]
  26. Sanderson C. J., Glauert A. M. The mechanism of T cell mediated cytotoxicity. V. Morphological studies by electron microscopy. Proc R Soc Lond B Biol Sci. 1977 Sep 5;198(1132):315–323. doi: 10.1098/rspb.1977.0100. [DOI] [PubMed] [Google Scholar]
  27. Santachiara A. S., Nabholz M., Miggiano V., Darlington A. J., Bodmer W. Genetic analysis with man-mouse somatic cell hybrids. Linkage between human lactate dehydrogenase B and peptidase B genes. Nature. 1970 Jul 18;227(5255):248–251. doi: 10.1038/227248a0. [DOI] [PubMed] [Google Scholar]
  28. Stejskal V., Holm G., Perlmann P. Differential cytotoxicity of activated lymphocytes on allogeneic and xenogeneic target cells. I. Activation by tuberculin and by Staphylococcus filtrate. Cell Immunol. 1973 Jul;8(1):71–81. doi: 10.1016/0008-8749(73)90094-4. [DOI] [PubMed] [Google Scholar]
  29. Wiig J. N. Effect of neuraminidase on lymphoid cells. Differences in structure of B and T cells and thymocytes of the mouse shown by cell electrophoresis and sialic acid determination. Scand J Immunol. 1974;3(3):357–363. doi: 10.1111/j.1365-3083.1974.tb01265.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES