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. 1978 May;75(5):2421–2423. doi: 10.1073/pnas.75.5.2421

Restricted classes of immunoglobulin produced by a lymphoid cell line from a patient with agammaglobulinemia.

J Schwaber, H Lazarus, F S Rosen
PMCID: PMC392565  PMID: 209464

Abstract

Restricted expression of immunoglobulin by a long-term B lymphoid cell line derived from a patient with X-linked agammaglobulinemia is reproted. The patient had peripheral blood B lymphocytes with surface IgD and IgM. Culture of the B cells in vitro with mitogens did not stimulate immunoglobulin secretion, although pokeweed mitogen stimulation resulted in the development of cytoplasmic IgD and IgM. The lymphoid cell line established from these B lymphocytes primarily bore surface IgD, with a small population of cells also bearing surface IgM. These cells also had cytoplasmic immunoglobulin, primarily IgD. The cell line did not have a cytoplasmic pool of immunoglobulin for export and did not secrete immunoglobulin. This B lymphoid cell line is an in vitro analogue of the arrest in differentiation of the patient's B lymphocytes. The IgD-bearing phenotype may represent a normal transitional stage in the differentiation of B lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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