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. 1978 May;75(5):2424–2428. doi: 10.1073/pnas.75.5.2424

Studies on the murine Ss protein: demonstration that the Ss protein is functionally the fourth component of complement.

M C Carroll, J D Capra
PMCID: PMC392566  PMID: 276882

Abstract

This report describes a simple and reproducible assay for the fourth component of murine complement (C4). Utilizing this assay, experiments are described which conclusively demonstrate that the murine Ss protein (serum substance) is functional C4 and that ascitic fluid may be used as a source for isolating Ss. The evidence that Ss is functional C4 is: (i) pretreatment of mouse plasma with F(ab')2 anti-Ss completely abrogated the C4 hemolytic titer; and (ii) in the isolation of the Ss protein from ascitic fluid, Ss immunochemical activity and C4 functional activity coeluted in each step of a four-step purification procedure.

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Selected References

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