Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 May;75(5):2424–2428. doi: 10.1073/pnas.75.5.2424

Studies on the murine Ss protein: demonstration that the Ss protein is functionally the fourth component of complement.

M C Carroll, J D Capra
PMCID: PMC392566  PMID: 276882

Abstract

This report describes a simple and reproducible assay for the fourth component of murine complement (C4). Utilizing this assay, experiments are described which conclusively demonstrate that the murine Ss protein (serum substance) is functional C4 and that ascitic fluid may be used as a source for isolating Ss. The evidence that Ss is functional C4 is: (i) pretreatment of mouse plasma with F(ab')2 anti-Ss completely abrogated the C4 hemolytic titer; and (ii) in the isolation of the Ss protein from ascitic fluid, Ss immunochemical activity and C4 functional activity coeluted in each step of a four-step purification procedure.

Full text

PDF
2424

Images in this article

2427Image
on p.2427
2427Image
on p.2427

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolotin C., Morris S., Tack B., Prahl J. Purification and structural analysis of the fourth component of human complement. Biochemistry. 1977 May 3;16(9):2008–2015. doi: 10.1021/bi00628a039. [DOI] [PubMed] [Google Scholar]
  2. Budzko D. B., Müller-Eberhard H. Anaphylatoxin release from the third component of human complement by hydroxylamine. Science. 1969 Aug 1;165(3892):506–507. doi: 10.1126/science.165.3892.506. [DOI] [PubMed] [Google Scholar]
  3. Capra J. D., Vitetta E. S., Klein J. Studies on the murine Ss protein. I. Purification, molecular weight, and subunit structure. J Exp Med. 1975 Sep 1;142(3):664–672. doi: 10.1084/jem.142.3.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Curman B., Ostberg L., Sandberg L., Malmheden-Eriksson I., Stålenheim G., Rask L., Peterson P. A. H-2 linked Ss protein is C4 component of complement. Nature. 1975 Nov 20;258(5532):243–245. doi: 10.1038/258243a0. [DOI] [PubMed] [Google Scholar]
  5. Démant P., Capková J., Hinzová E., Vorácová B. The role of the histocompatibility-2-linked Ss-Slp region in the control of mouse complement. Proc Natl Acad Sci U S A. 1973 Mar;70(3):863–864. doi: 10.1073/pnas.70.3.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gaither T. A., Alling D. W., Frank M. M. A new one-step method for the functional assay of the fourth component (C4) of human and guinea pig complement. J Immunol. 1974 Aug;113(2):574–583. [PubMed] [Google Scholar]
  7. Goldman M. B., Goldman J. N. Relationship of functional levels of early components of complement to the H-2 complex of mice. J Immunol. 1976 Nov;117(5 Pt 1):1584–1588. [PubMed] [Google Scholar]
  8. Gordon J., Whitehead H. R., Wormall A. The Action of Ammonia on Complement. The Fourth Component. Biochem J. 1926;20(5):1028–1035. doi: 10.1042/bj0201028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hansen T. H., Shin H. S., Shreffler D. C. Evidence for the involvement of the Ss protein of the mouse in the hemolytic complement system. J Exp Med. 1975 May 1;141(5):1216–1220. doi: 10.1084/jem.141.5.1216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hinzová E., Démant P., Iványi P. Genetic control of haemolytic complement in mice: association with H-2. Folia Biol (Praha) 1972;18(4):237–243. [PubMed] [Google Scholar]
  11. Lachmann P. J., Grennan D., Martin A., Demant P. Identification of Ss protein as murine C4. Nature. 1975 Nov 20;258(5532):242–243. doi: 10.1038/258242a0. [DOI] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  14. Mandle R. J., Jr, McConnell-Mapes J. A., Nilsson U. R. Inhibition of complement by mouse serum: selective inactivation of the fourth component of human complement. J Immunol. 1977 Jul;119(1):180–186. [PubMed] [Google Scholar]
  15. Meo T., Krasteff T., Shreffler D. C. Immunochemical characterization of murine H-2 controlled Ss (serum substance) protein through identification of its human homologue as the fourth component of complement. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4536–4540. doi: 10.1073/pnas.72.11.4536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Müller-Eberhard H. J. Complement. Annu Rev Biochem. 1969;38:389–414. doi: 10.1146/annurev.bi.38.070169.002133. [DOI] [PubMed] [Google Scholar]
  17. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  18. Pangburn M. K., Schreiber R. D., Müller-Eberhard H. J. Human complement C3b inactivator: isolation, characterization, and demonstration of an absolute requirement for the serum protein beta1H for cleavage of C3b and C4b in solution. J Exp Med. 1977 Jul 1;146(1):257–270. doi: 10.1084/jem.146.1.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Passmore H. C., Shreffler D. C. A sex-limited serum protein variant in the mouse: inheritance and association with the H-2 region. Biochem Genet. 1970 Jun;4(3):351–365. doi: 10.1007/BF00485752. [DOI] [PubMed] [Google Scholar]
  20. Schreiber R. D., Müller-Eberhard H. J. Fourth component of human complement: description of a three polypeptide chain structure. J Exp Med. 1974 Nov 1;140(5):1324–1335. doi: 10.1084/jem.140.5.1324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shreffler D C, Owen R D. A Serologically Detected Variant in Mouse Serum: Inheritance and Association with the Histocompatibility-2 Locus. Genetics. 1963 Jan;48(1):9–25. doi: 10.1093/genetics/48.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shreffler D. C. The S region of the mouse major histocompatibility complex (H-2): genetic variation and functional role in complement system. Transplant Rev. 1976;32:140–167. doi: 10.1111/j.1600-065x.1976.tb00232.x. [DOI] [PubMed] [Google Scholar]
  23. Tung A. S., Ju S. T., Sato S., Nisonoff A. Production of large amounts of antibodies in individual mice. J Immunol. 1976 Mar;116(3):676–681. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES