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. 1978 May;75(5):2443–2447. doi: 10.1073/pnas.75.5.2443

Use of anti-idiotypic antibodies as cell-surface receptor probes

Karin Sege 1, Per A Peterson 1
PMCID: PMC392570  PMID: 307766

Abstract

Anti-idiotypic antibodies have been raised against antibodies to retinol-binding protein (RBP) and to insulin. After absorption the anti-idiotypic antibodies recognized the antigen-combining sites of the antibodies used as the immunogen but of no other antibodies. Some of the anti-idiotypic antibodies raised against antibodies to RBP bound specifically to rat intestine epithelial cells, which have a physiological cell-surface receptor for RBP. The RBP receptor mediates the uptake of retinol from RBP to the cells. This uptake was abolished in a concentration-dependent manner by the anti-idiotypic antibodies, which obviously competed with RBP for binding to the receptor.

Anti-idiotypic antibodies against antibodies to insulin inhibited the binding of 125I-labeled insulin to isolated rat epididymal fat cells, whereas anti-idiotypic antibodies raised against antibodies to RBP had no effect. Furthermore, on interacting with young rat thymocytes, anti-idiotypic antibodies against antibodies to insulin stimulated the uptake by the cells of α-aminoisobutyric acid, thereby mimicking the effect of insulin.

These results suggest that in some cases anti-idiotypic antibodies may be useful tools in elucidating structure-function relationships for cell-membrane receptors.

Keywords: retinol-binding protein, insulin receptor, insulin effect

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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