Table 1. Oligonucleoide primers and fluorescent probes used to quantitate mRNA.
Gene | Paired primers (5′-3′) | Probea (5′-3′) |
---|---|---|
emm | AGACAGTTAC CATCAACAGG TGAAAC TACTCCAGCT GTTGCCATAA CAG |
TAACCCATTC TTCACAGCGG CAGCC |
hasA | ACCGTTCCCT TGTCAATAAA GG CGTCAGCGTC AGATCTTTCA AA |
CGCCATGCTC AAGCGTGGGC |
mga | GCGTTTGATA GCATCAAACA AGA CATCAAGGAG ATGAACCCAG TTG |
TCACTTTTCG ACAGCCCGTT GGTGA |
gyrA | CGACTTGTCT GAACGCCAAA TTATCACGTT CCAAACCAGT CAA |
CGACGCAAAC GCATATCCAA AATAGCTTG |
a Covalently linked at the 5′ end to 5-carboxylfluorescein and at the 3′ end to N,N,N-tetramethyl-6-carboxyrhodamine.