Abstract
Postmortem study of proliferative glomerulonephritis associated with human systemic lumpus has previously shown that an antigen related to mammalian type C RNA viral core (p30) proteins is deposited in the renal glomerular lesions with human immunoglobulins in an immune-complex pattern. In the present work, human immunoglobulins were sequentially eluted from the lupus glomerular immune deposits and were assayed by a sensitive enzymoimmunoassay developed for the measurement of anti-p30 antibody activity against purified viral p30 proteins of mammalian type C viruses. Human immunoglobulins showing specific anti-p30 antibody activity, particularly against p30 antigen of feline endogenous virus RD-114 and to a smaller extent against p30 antigen of murine type C virus, were eluted by acid buffer from the glomerular immune deposits in two patients with lupus proliferative glomerulonephritis who have deposits of viral p30-related antigen in the same tissue lesions. This study adds support for the hypothesis that expression of type C viral antigen may be involved in the multifactorial pathogenesis of proliferative glomerulonephritis associated with human systemic lupus.
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Selected References
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- Aoki T., Walling M. J., Bushar G. S., Liu M., Hsu K. C. Natural antibodies in sera from healthy humans to antigens on surfaces of type C RNA viruses and cells from primates. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2491–2495. doi: 10.1073/pnas.73.7.2491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
- Benveniste R. E., Todaro G. J. Evolution of type C viral genes: I. Nucleic acid from baboon type C virus as a measure of divergence among primate species. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4513–4518. doi: 10.1073/pnas.71.11.4513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Charman H. P., Kim N., White M., Gilden R. V. Failure to detect, in human sera, antibodies cross-reactive with group-specific antigens of murine leukemia virus. J Natl Cancer Inst. 1974 May;52(5):1409–1413. doi: 10.1093/jnci/52.5.1409. [DOI] [PubMed] [Google Scholar]
- Clark G., Reichlin M., Tomasi T. B., Jr Characterization of a soluble cytoplasmic antigen reactive with sera from patients with systemic lupus erythmatosus. J Immunol. 1969 Jan;102(1):117–122. [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
- Gallagher R. E., Gallo R. C. Type C RNA tumor virus isolated from cultured human acute myelogenous leukemia cells. Science. 1975 Jan 31;187(4174):350–353. doi: 10.1126/science.46123. [DOI] [PubMed] [Google Scholar]
- Geering G., Hardy W. D., Jr, Old L. J., de Harven E., Brodey R. S. Shared group-specific antigen of murine and feline leukemia viruses. Virology. 1968 Dec;36(4):678–680. doi: 10.1016/0042-6822(68)90199-2. [DOI] [PubMed] [Google Scholar]
- Geering G., Old L. J., Boyse E. A. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J Exp Med. 1966 Oct 1;124(4):753–772. doi: 10.1084/jem.124.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilden R. V., Oroszlan S., Huebner R. J. Coexistence of intraspecies and interspecies specific antigenic determinants on the major structural polypeptide of mammalian C-type viruses. Nat New Biol. 1971 May 26;231(21):107–108. doi: 10.1038/newbio231107a0. [DOI] [PubMed] [Google Scholar]
- Gilden R. V., Toni R., Hanson M., Bova D., Charman H. P., Oroszlan S. Immunochemical studies of the major internal polypeptide of woolly monkey and gibbon ape type C viruses. J Immunol. 1974 Mar;112(3):1250–1254. [PubMed] [Google Scholar]
- HUEBNER R. J., ARMSTRONG D., OKUYAN M., SARMA P. S., TURNER H. C. SPECIFIC COMPLEMENT-FIXING VIRAL ANTIGENS IN HAMSTER AND GUINEA PIG TUMORS INDUCED BY THE SCHMIDT-RUPPIN STRAIN OF AVIAN SARCOMA. Proc Natl Acad Sci U S A. 1964 May;51:742–750. doi: 10.1073/pnas.51.5.742. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hehlmann R., Kufe D., Spiegelman S. RNA in human leukemic cells related to the RNA of a mouse leukemia virus (leukocytes-RNA-DNA hybridization-rauscher virus-polysomal RNA). Proc Natl Acad Sci U S A. 1972 Feb;69(2):435–439. doi: 10.1073/pnas.69.2.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hilgers J., Nowinski R. C., Geering G., Hardy W. Detection of avian and mammalian oncogenic RNA viruses (oncornaviruses) by immunofluorescence. Cancer Res. 1972 Jan;32(1):98–106. [PubMed] [Google Scholar]
- Koffler D., Agnello V., Kimkel H. G. Polynucleotide immune complexes in serum and glomeruli of patients with systemic lupus erythematosus. Am J Pathol. 1974 Jan;74(1):109–124. [PMC free article] [PubMed] [Google Scholar]
- Kurth R., Teich N. M., Weiss R., Oliver R. T. Natural human antibodies reactive with primate type-C viral antigens. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1237–1241. doi: 10.1073/pnas.74.3.1237. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lewis R. M., Tannenberg W., Smith C., Schwartz R. S. C-type viruses in systemic lupus erythematosus. Nature. 1974 Nov 1;252(5478):78–79. doi: 10.1038/252078a0. [DOI] [PubMed] [Google Scholar]
- Louie S., Curtis J. E., Till J. E., McCulloch E. A. Antibodies in human sera to oncorna virus-like proteins from normal or leukemia marrow cell cultures. J Exp Med. 1976 Nov 2;144(5):1243–1253. doi: 10.1084/jem.144.5.1243. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mattioli M., Reichlin M. Heterogeneity of RNA protein antigens reactive with sera of patients with systemic lupus erythematosus. Description of a cytoplasmic nonribosomal antigen. Arthritis Rheum. 1974 Jul-Aug;17(4):421–429. doi: 10.1002/art.1780170413. [DOI] [PubMed] [Google Scholar]
- Mellors R. C., Aoki T., Huebner R. J. Further implication of murine leukemia-like virs in the disorders of NZB mice. J Exp Med. 1969 May 1;129(5):1045–1062. doi: 10.1084/jem.129.5.1045. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mellors R. C., Mellors J. W. Antigen related to mammalian type-C RNA viral p30 proteins is located in renal glomeruli in human systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1976 Jan;73(1):233–237. doi: 10.1073/pnas.73.1.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nooter K., Aarssen A. M., Bentvelzen P., De Groot F. G., Van Pelt F. G. Isolation of infectious C-type oncornavirus from human leukaemic bone marrow cells. Nature. 1975 Aug 14;256(5518):595–597. doi: 10.1038/256595a0. [DOI] [PubMed] [Google Scholar]
- Oroszlan S., Huebner R. J., Gilden R. V. Species-specific and interspecific antigenic determinants associated with the structural protein of feline C-type virus. Proc Natl Acad Sci U S A. 1971 May;68(5):901–904. doi: 10.1073/pnas.68.5.901. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Panem S., Ordóez N. G., Kirstein W. H., Katz A. I., Spargo B. H. C-type virus expression in systemic lupus erythematosus. N Engl J Med. 1976 Aug 26;295(9):470–475. doi: 10.1056/NEJM197608262950903. [DOI] [PubMed] [Google Scholar]
- Panem S., Prochownik E. V., Reale F. R., Kirsten W. H. Isolation of type C virions from a normal human fibroblast strain. Science. 1975 Jul 25;189(4199):297–299. doi: 10.1126/science.49927. [DOI] [PubMed] [Google Scholar]
- Parks W. P., Scolnick E. M. Radioimmunoassay of mammalian type-C viral proteins: interspecies antigenic reactivities of the major internal polypeptide. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1766–1770. doi: 10.1073/pnas.69.7.1766. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reitz M. S., Miller N. R., Wong-Staal F., Gallagher R. E., Gallo R. C., Gillespie D. H. Primate type-C virus nucleic acid sequences (woolly monkey and baboon types) in tissues from a patient with acute myelogenous leukemia and in viruses isolated from cultured cells of the same patient. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2113–2117. doi: 10.1073/pnas.73.6.2113. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sherr C. J., Fedele L. A., Benveniste R. E., Todaro G. J. Interspecies antigenic determinants of the reverse transcriptases and p30 proteins of mammalian type C viruses. J Virol. 1975 Jun;15(6):1440–1448. doi: 10.1128/jvi.15.6.1440-1448.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sherr C. J., Todaro G. J. Radioimmunoassay of the major group specific protein of endogenous baboon type C viruses: relation to the RD-114-CCC group and detection of antigen in normal baboon tissues. Virology. 1974 Sep;61(1):168–181. doi: 10.1016/0042-6822(74)90252-9. [DOI] [PubMed] [Google Scholar]
- Sherr C. J., Todaro G. J. Type C viral antigens in man. I. Antigens related to endogenous primate virus in human tumors. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4703–4707. doi: 10.1073/pnas.71.12.4703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Snyder H. W., Jr, Pincus T., Fleissner E. Specificities of human immunoglobulins reactive with antigens in preparations of several mammalian type-C viruses. Virology. 1976 Nov;75(1):60–73. doi: 10.1016/0042-6822(76)90007-6. [DOI] [PubMed] [Google Scholar]
- Stephenson J. R., Aaronson S. A. Expression of endogenous RNA C-type virus group-specific antigens in mammalian cells. J Virol. 1973 Sep;12(3):564–569. doi: 10.1128/jvi.12.3.564-569.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stephenson J. R., Aaronson S. A. Search for antigens and antibodies crossreactive with type C viruses of the woolly monkeys and gibbon ape in animal models and in humans. Proc Natl Acad Sci U S A. 1976 May;73(5):1725–1729. doi: 10.1073/pnas.73.5.1725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strand M., August J. T. Structural proteins of mammalian RNA tumor viruses: relatedness of the interspecies antigenic determinants of the major internal protein. J Virol. 1975 Jun;15(6):1332–1341. doi: 10.1128/jvi.15.6.1332-1341.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strand M., August J. T. Type-C RNA virus gene expression in human tissue. J Virol. 1974 Dec;14(6):1584–1596. doi: 10.1128/jvi.14.6.1584-1596.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sutherland J. C., Mardiney M. R., Jr Immune complex disease in the kidneys of lymphoma-leukemia patients: the presence of an oncornavirus-related antigen. J Natl Cancer Inst. 1973 Mar;50(3):633–644. doi: 10.1093/jnci/50.3.633. [DOI] [PubMed] [Google Scholar]
- Yoshiki T., Mellors R. C., Strand M., August J. T. The viral envelope glycoprotein of murine leukemia virus and the pathogenesis of immune complex glomerulonephritis of New Zealand mice. J Exp Med. 1974 Oct 1;140(4):1011–1027. doi: 10.1084/jem.140.4.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]