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. 1978 May;75(5):2525–2529. doi: 10.1073/pnas.75.5.2525

Different time course of development for high-affinity choline uptake and choline acetyltransferase in the chick retina.

C R Bader, R W Baughman, J L Moore
PMCID: PMC392587  PMID: 276888

Abstract

Synthesis and storage of [3H]acetylcholine in isolated pieces of chick retina increased in two stages during embryogenesis. The first increase coincided with a 100-fold rise in the activity of choline acetyltransferase (acetyl-CoA:choline O-acetyltransferase, EC 2.3.1.6), but during the second increase the activity of this enzyme remained essentially constant. The second increase instead was linked to an approximately 6-fold increase in the Vmax for high-affinity uptake of choline.

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Selected References

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  1. Barker L. A., Mittag T. W. Comparative studies of substrates and inhibitors of choline transport and choline acetyltransferase. J Pharmacol Exp Ther. 1975 Jan;192(1):86–94. [PubMed] [Google Scholar]
  2. Baughman R. W., Bader C. R. Biochemical characterization and cellular localization of the cholinergic system in the chicken retina. Brain Res. 1977 Dec 23;138(3):469–485. doi: 10.1016/0006-8993(77)90684-9. [DOI] [PubMed] [Google Scholar]
  3. Burt A. M. Acetylcholinesterase and choline acetyltransferase activity in the developing chick spinal cord. J Exp Zool. 1968 Sep;169(1):107–112. doi: 10.1002/jez.1401690112. [DOI] [PubMed] [Google Scholar]
  4. COULOMBRE A. J. Correlations of structural and biochemical changes in the developing retina of the chick. Am J Anat. 1955 Jan;96(1):153–189. doi: 10.1002/aja.1000960106. [DOI] [PubMed] [Google Scholar]
  5. Chiappinelli V., Giacobini E., Pilar G., Uchimura H. Induction of cholinergic enzymes in chick ciliary ganglion and iris muscle cells during synapse formation. J Physiol. 1976 Jun;257(3):749–766. doi: 10.1113/jphysiol.1976.sp011395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Coyle J. T., Axelrod J. Development of the uptake and storage of L-( 3 H)norepinephrine in the rat brain. J Neurochem. 1971 Nov;18(11):2061–2075. doi: 10.1111/j.1471-4159.1971.tb05065.x. [DOI] [PubMed] [Google Scholar]
  7. Coyle J. T., Enna S. J. Neurochemical aspects of the ontogenesis of GABAnergic neurons in the rat brain. Brain Res. 1976 Jul 23;111(1):119–133. doi: 10.1016/0006-8993(76)91053-2. [DOI] [PubMed] [Google Scholar]
  8. Coyle J. T., Yamamura H. I. Neurochemical aspects of the ontogenesis of cholinergic neurons in the rat brain. Brain Res. 1976 Dec 24;118(3):429–440. doi: 10.1016/0006-8993(76)90310-3. [DOI] [PubMed] [Google Scholar]
  9. Fonnum F. A rapid radiochemical method for the determination of choline acetyltransferase. J Neurochem. 1975 Feb;24(2):407–409. doi: 10.1111/j.1471-4159.1975.tb11895.x. [DOI] [PubMed] [Google Scholar]
  10. GRAY E. G., WHITTAKER V. P. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J Anat. 1962 Jan;96:79–88. [PMC free article] [PubMed] [Google Scholar]
  11. Glover V. A., Potter L. T. Purification and properties of choline acetyltransferase from ox brain striate nuclei. J Neurochem. 1971 Apr;18(4):571–580. doi: 10.1111/j.1471-4159.1971.tb11987.x. [DOI] [PubMed] [Google Scholar]
  12. HEBB C. O. Biochemical evidence for the neural function of acetylcholine. Physiol Rev. 1957 Apr;37(2):196–220. doi: 10.1152/physrev.1957.37.2.196. [DOI] [PubMed] [Google Scholar]
  13. Hildebrand J. G., Barker D. L., Herbert E., Kravitz E. A. Screening for neurotransmitters: a rapid radiochemical procedure. J Neurobiol. 1971;2(3):231–246. doi: 10.1002/neu.480020305. [DOI] [PubMed] [Google Scholar]
  14. Hughes W. F., LaVelle A. On the synaptogenic sequence in the chick retina. Anat Rec. 1974 Jul;179(3):297–301. doi: 10.1002/ar.1091790302. [DOI] [PubMed] [Google Scholar]
  15. Kahn A. J. An autoradiographic analysis of the time of appearance of neurons in the developing chick neural retina. Dev Biol. 1974 May;38(1):30–40. doi: 10.1016/0012-1606(74)90256-5. [DOI] [PubMed] [Google Scholar]
  16. Kaita A. A., Goldberg A. M. Control of acetylcholine synthesis--the inhibition of choline acetyltransferase by acetylcholine. J Neurochem. 1969 Jul;16(7):1185–1191. doi: 10.1111/j.1471-4159.1969.tb05964.x. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. MCCAMAN R. E., HUNT J. M. MICRODETERMINATION OF CHOLINE ACETYLASE IN NERVOUS TISSUE. J Neurochem. 1965 Apr;12:253–259. doi: 10.1111/j.1471-4159.1965.tb06762.x. [DOI] [PubMed] [Google Scholar]
  19. Rager G. Morphogenesis and physiogenesis of the retino-tectal connection in the chicken. II. The retino-tectal synapses. Proc R Soc Lond B Biol Sci. 1976 Feb 17;192(1108):353–370. doi: 10.1098/rspb.1976.0018. [DOI] [PubMed] [Google Scholar]
  20. Ross C. D., McDougal D. B., Jr The distribution of choline acetyltransferase activity in vertebrate retina. J Neurochem. 1976 Mar;26(3):521–526. doi: 10.1111/j.1471-4159.1976.tb01505.x. [DOI] [PubMed] [Google Scholar]
  21. SHEN S. C., GREENFIELD P., BOELL E. J. Localization of acetylcholinesterase in chick retina during histogenesis. J Comp Neurol. 1956 Dec;106(2):433–461. doi: 10.1002/cne.901060211. [DOI] [PubMed] [Google Scholar]
  22. Sanes J. R., Hildebrand J. G. Acetylcholine and its metabolic enzymes in developing antennae of the moth, Manduca sexta. Dev Biol. 1976 Aug;52(1):105–120. doi: 10.1016/0012-1606(76)90011-7. [DOI] [PubMed] [Google Scholar]
  23. Simon J. R., Atweh S., Kuhar M. J. Sodium-dependent high affinity choline uptake: a regulatory step in the synthesis of acetylcholine. J Neurochem. 1976 May;26(5):909–922. doi: 10.1111/j.1471-4159.1976.tb06472.x. [DOI] [PubMed] [Google Scholar]
  24. Sorimachi M., Kataoka K. High affinity choline uptake: an early index of cholinergic innervation in rat brain. Brain Res. 1975 Aug 29;94(2):325–336. doi: 10.1016/0006-8993(75)90065-7. [DOI] [PubMed] [Google Scholar]
  25. Suszkiw J. B., Pilar G. Selective localization of a high affinity choline uptake system and its role in ACh formation in cholinergic nerve terminals. J Neurochem. 1976 Jun;26(6):1133–1138. doi: 10.1111/j.1471-4159.1976.tb06996.x. [DOI] [PubMed] [Google Scholar]
  26. Vogel Z., Nirenberg M. Localization of acetylcholine receptors during synaptogenesis in retina. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1806–1810. doi: 10.1073/pnas.73.6.1806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WITKOVSKY P. AN ONTOGENETIC STUDY OF RETINAL FUNCTION IN THE CHICK. Vision Res. 1963 Nov;61:341–355. doi: 10.1016/0042-6989(63)90008-7. [DOI] [PubMed] [Google Scholar]
  28. Wang G. K., Schmidt J. Receptors for alpha-bungarotoxin in the developing visual system of the chick. Brain Res. 1976 Sep 24;114(3):524–529. doi: 10.1016/0006-8993(76)90976-8. [DOI] [PubMed] [Google Scholar]
  29. White H. L., Cavallito C. J. Photoisomerization of styrylpyridine analogues in relation to choline acetyltransferase and cholinesterase inhibition. Biochim Biophys Acta. 1970 May 13;206(2):242–251. doi: 10.1016/0005-2744(70)90107-5. [DOI] [PubMed] [Google Scholar]
  30. White H. L., Wu J. C. Choline and carnitine acetyltransferases of heart. Biochemistry. 1973 Feb 27;12(5):841–846. doi: 10.1021/bi00729a009. [DOI] [PubMed] [Google Scholar]
  31. White H. L., Wu J. C. Kinetics of choline acetyltransferases (EC 2.3.1.6) from human and other mammalian central and peripheral nervous tissues. J Neurochem. 1973 Feb;20(2):297–307. doi: 10.1111/j.1471-4159.1973.tb12129.x. [DOI] [PubMed] [Google Scholar]

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