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. 1978 Jun;75(6):2654–2658. doi: 10.1073/pnas.75.6.2654

Elimination of cooperativity in aspartate transcarbamylase by nitration of a single tyrosine residue.

S M Landfear, D R Evans, W N Lipscomb
PMCID: PMC392621  PMID: 26914

Abstract

In a previous report [Landfear, S. M., Lipscomb, W. N. & Evans, D.R. (1978) J. Biol. Chem. 253, 3988--3996] we demonstrated that tetranitromethane can be employed to nitrate a limited number of tyrosine residues in aspartate transcarbamylase (carbamoylphosphate:L-aspartate carbamoyltransferase, EC 2.1.3.2); such modification eliminates cooperativity, feedback inhibition, and enzymatic activity, and reduces binding of the feedback inhibitor cytidine triphosphate. Cooperativity is lost more rapidly than other properties, and this loss correlates with the nitration of a single tyrosine residue. In this paper, we describe the saturation kinetics of hybrid species constructed from nitrated subunits of one type (either catalytic or regulatory) and native subunits of the other type. We conclude that the modification responsible for loss of cooperativity is on the catalytic subunit. The tryptic peptide containing this modification has been isolated and identified.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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