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. 1978 Jun;75(6):2776–2780. doi: 10.1073/pnas.75.6.2776

In vitro differentiation of a fast Na+ conductance in embryonic heart cell aggregates.

R D Nathan, R L DeHaan
PMCID: PMC392647  PMID: 275846

Abstract

Aggregates of 3-day chick cardiac myocytes, after 2 days of gyration culture, lack a tetrodotoxin-sensitive, fast Na+ conductance. After 2--3 additional days in vitro these aggregates were shown by three different criteria to have developed such a conductance mechanism: (a) action potential upstroke velocities greater than 90 V/sec; (b) suppression of spontaneous activity by tetrodotoxin; and (c) presence of a fast component of inward current recorded during voltage clamp. These membrane properties were similar to those of aggregates derived from 7-day hearts that had developed in ovo. The differentiation of fast Na+ conductance channels was a dependent upon active protein synthesis but not upon the presence of exogenous macromolecules.

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Selected References

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