Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 Jun;75(6):2893–2896. doi: 10.1073/pnas.75.6.2893

Bone marrow colony-stimulating factor and tumor resistance-enhancing activity of postendotoxin mouse sera

R Christopher Butler 1,*, Alexander M Abdelnoor 1,†, Alois Nowotny 1,‡
PMCID: PMC392671  PMID: 351621

Abstract

The passive transfer of postendotoxin mouse serum could enhance nonspecific resistance to the development of TA3-Ha transplantable ascites tumor in mice. The postendotoxin serum was not directly cytotoxic to TA3-Ha tumor cells in vitro, nor did it contain significant amounts of residual endotoxin, but it was rich in colony-stimulating factors (CSFs). High-titer CSF serum could be induced by endotoxic lipopolysaccharide (LPS). Nonendotoxic, lipid-free, and polysaccharide-rich hydrolytic breakdown product of LPS (called PS) was less potent but still active in CSF induction. There was a correlation between the level of CSF stimulation and the capacity of the sera to transfer tumor resistance (TUR). Those LPS preparations that had the highest CSF-inducing capacity were the most potent in TUR enhancement. Suppression of CSF production by treatment with theophylline or epinephrine, enhancers of cyclic AMP/cyclic GMP ratios, lowered the enhancement of TUR by endotoxic LPS. The infection of serum donor mice with bacillus Calmette-Guérin (BCG) 18 days prior to LPS treatment gave the highest serum CSF levels and the most potent TUR-inducing serum preparation. Even more notable was the finding that the nontoxic PS preparation could replace toxic LPS in the above BCG-LPS system. The serum harvested from BCG-infected mice 2 hr after PS injection was similarly effective in the passive transfer of TUR.

Keywords: TA3-Ha ascites tumor, passive transfer, lipopolysaccharide, tumor-necrotizing factor, anti-tumor resistance

Full text

PDF
2893

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradley T. R., Metcalf D. The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci. 1966 Jun;44(3):287–299. doi: 10.1038/icb.1966.28. [DOI] [PubMed] [Google Scholar]
  2. Carswell E. A., Old L. J., Kassel R. L., Green S., Fiore N., Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3666–3670. doi: 10.1073/pnas.72.9.3666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chervenick P. A. Effect of endotoxin and postendotoxin plasma on in vitro granulopoiesis. J Lab Clin Med. 1972 Jun;79(6):1014–1020. [PubMed] [Google Scholar]
  4. Golde D. W., Cline M. J. Endotoxin-induced release of colony-stimulating activity in man. Proc Soc Exp Biol Med. 1975 Sep;149(4):845–848. doi: 10.3181/00379727-149-38911. [DOI] [PubMed] [Google Scholar]
  5. ISAACS A. INTERFERON. Adv Virus Res. 1963;10:1–38. [PubMed] [Google Scholar]
  6. Kampschmidt R. F., Pulliam L. A. Stimulation of antimicrobial activity in the rat with leukocytic endogenous mediator. J Reticuloendothel Soc. 1975 Mar;17(3):162–169. [PubMed] [Google Scholar]
  7. Levin J., Poore T. E., Zauber N. P., Oser R. S. Detection of endotoxin in the blood of patients with sepsis due to gran-negative bacteria. N Engl J Med. 1970 Dec 10;283(24):1313–1316. doi: 10.1056/NEJM197012102832404. [DOI] [PubMed] [Google Scholar]
  8. Nowotny A., Behling U. H., Chang H. L. Relation of structure to function in bacterial endotoxins. VIII. Biological activities in a polysaccharide-rich fraction. J Immunol. 1975 Jul;115(1):199–203. [PubMed] [Google Scholar]
  9. Nowotny A., Golub S., Key B. Fate and effect of endotoxin derivtives in tumor-bearing mice. Proc Soc Exp Biol Med. 1971 Jan;136(1):66–69. doi: 10.3181/00379727-136-35194. [DOI] [PubMed] [Google Scholar]
  10. Quesenberry P., Morley A., Stohlman F., Jr, Rickard K., Howard D., Smith M. Effect of endotoxin on granulopoiesis and colony-stimulating factor. N Engl J Med. 1972 Feb 3;286(5):227–232. doi: 10.1056/NEJM197202032860502. [DOI] [PubMed] [Google Scholar]
  11. SMITH R. T., THOMAS L. The lethal effect of endotoxins on the chick embryo. J Exp Med. 1956 Aug 1;104(2):217–231. doi: 10.1084/jem.104.2.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SUTER E., ULLMAN G. E., HOFFMAN R. G. Sensitivity of mice to endotoxin after vaccination with BCG (Bacillus Calmette-Guérin). Proc Soc Exp Biol Med. 1958 Oct;99(1):167–169. doi: 10.3181/00379727-99-24282. [DOI] [PubMed] [Google Scholar]
  13. Seligman S. J. In vitro susceptibility of methicillin-resistant Staphylococcus aureus to sulfamethoxazole and trimethoprim. J Infect Dis. 1973 Nov;128(Suppl):543–p. doi: 10.1093/infdis/128.supplement_3.s543. [DOI] [PubMed] [Google Scholar]
  14. Stanley E. R., Hansen G., Woodcock J., Metcalf D. Colony stimulating factor and the regulation of granulopoiesis and macrophage production. Fed Proc. 1975 Dec;34(13):2272–2278. [PubMed] [Google Scholar]
  15. Yang C., Nowotny A. Effect of endotoxin on tumor resistance in mice. Infect Immun. 1974 Jan;9(1):95–100. doi: 10.1128/iai.9.1.95-100.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES