Abstract
Using [3H]quinuclidinyl benzilate as a specific cholinergic muscarinic ligand, it has been demonstrated that lymphocytes have muscarinic binding sites. There are approximately 200 sites per cell and the dissociation constant for quinuclidinyl benzilate is approximately 1 X 10(-9) M. Quinuclidinyl benzilate receptor binding is blocked by atropine and oxotremorine.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aronstam R. S., Abood L. G., MacNeil M. K. Muscarinic cholinergic binding in human erythrocyte membranes. Life Sci. 1977 Apr 1;20(7):1175–1180. doi: 10.1016/0024-3205(77)90489-1. [DOI] [PubMed] [Google Scholar]
- Birdsall N. J., Burgen A. S., Hiley C. R., Hulme E. C. Binding of agonists and antagonists to muscarinic receptors. J Supramol Struct. 1976;4(3):367–371. doi: 10.1002/jss.400040307. [DOI] [PubMed] [Google Scholar]
- Boyle W. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968 Sep;6(6):761–764. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
- Changeux J. P., Kasai M., Lee C. Y. Use of a snake venom toxin to characterize the cholinergic receptor protein. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1241–1247. doi: 10.1073/pnas.67.3.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen J. J., Fairchild S. S. T-Lymphocyte precursors. I. Synergy between precursor and mature T lymphocytes in the response to concanavalin A. J Exp Med. 1976 Aug 1;144(2):456–466. doi: 10.1084/jem.144.2.456. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EMMELIN N., MACINTOSH F. C. The release of acetylcholine from perfused sympathetic ganglia and skeletal muscles. J Physiol. 1956 Feb 28;131(2):477–496. doi: 10.1113/jphysiol.1956.sp005477. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fambrough D. M., Hartzell H. C. Acetylcholine receptors: number and distribution at neuromuscular junctions in rat diaphragm. Science. 1972 Apr 14;176(4031):189–191. doi: 10.1126/science.176.4031.189. [DOI] [PubMed] [Google Scholar]
- Illiano G., Tell G. P., Siegel M. E., Cuatrecasas P. Guanosine 3':5'-cyclic monophosphate and the action of insulin and acetylcholine. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2443–2447. doi: 10.1073/pnas.70.8.2443. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krug U., Krug F., Cuatrecasas P. Emergence of insulin receptors on human lymphocytes during in vitro transformation. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2604–2608. doi: 10.1073/pnas.69.9.2604. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miledi R., Potter L. T. Acetylcholine receptors in muscle fibres. Nature. 1971 Oct 29;233(5322):599–603. doi: 10.1038/233599a0. [DOI] [PubMed] [Google Scholar]
- PATON W. D., RANG H. P. THE UPTAKE OF ATROPINE AND RELATED DRUGS BY INTESTINAL SMOOTH MUSCLE OF THE GUINEA-PIG IN RELATION TO ACETYLCHOLINE RECEPTORS. Proc R Soc Lond B Biol Sci. 1965 Aug 24;163:1–44. doi: 10.1098/rspb.1965.0058. [DOI] [PubMed] [Google Scholar]
- Raftery M. A., Schmidt J., Clark D. G., Wolcott R. G. Demonstration of a specific -bungarotoxin binding component in electrophorus electricus electroplax membranes. Biochem Biophys Res Commun. 1971 Dec 17;45(6):1622–1629. doi: 10.1016/0006-291x(71)90207-5. [DOI] [PubMed] [Google Scholar]
- Strange P. G., Birdsall N. J., Burgen A. S. Occupancy of muscarinic acetylcholine receptors stimulates a guanylate cyclase in neuroblastoma cells. Biochem Soc Trans. 1977;5(1):189–191. doi: 10.1042/bst0050189. [DOI] [PubMed] [Google Scholar]
- Strom T. B., Sytkowski A. J., Carpenter C. B., Merrill J. P. Cholinergic augmentation of lymphocyte-mediated cytotoxicity. A study of the cholinergic receptor of cytotoxic T lymphocytes. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1330–1333. doi: 10.1073/pnas.71.4.1330. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamamura H. I., Snyder S. H. Muscarinic cholinergic binding in rat brain. Proc Natl Acad Sci U S A. 1974 May;71(5):1725–1729. doi: 10.1073/pnas.71.5.1725. [DOI] [PMC free article] [PubMed] [Google Scholar]