Skip to main content
. 2013 Oct 28;4(12):2317–2325. doi: 10.18632/oncotarget.1464

Figure 2. Effects of 5-Aza-dC and/or TSA on demethylation and re-expression of silenced HOXA11.

Figure 2

(A & B) Re-expression of silenced HOXA11 was examined by RT-PCR (A) and real-time PCR (B) in six lung cancer cell lines after treatment of the cells with 5-Aza-dC for 72 h. Compared to other cell lines, re-expression of HOXA11 is minimal in H226 and A549 cells. Plus and minus signs indicate the presence or absence of 5-Aza-dC treatment, respectively. (C) Demethylation of silenced HOXA11 was analyzed using MS-HRM assay after treatment of 5-Aza-dC for 72 h in 6 lung cancer cell lines. Red and blue lines indicate the statuses with and without treatment of 5-Aza-dC, respectively. (D) Heatmaps show typical patterns of demethylation: the degree of demethylation is high in H460 cells with less densely methylated HOXA11 and minimal in A549 cells with densely methylated HOXA11. (E & F) H226 and A549 cells that showed minimal re-expression of HOXA11 were treated with 10 M 5-Aza-dC in combination with 0.5 M TSA for 72 h. TSA induced additional re-expression of HOXA11 by RT-PCR (E) and real-time PCR (F). Error bars indicate one standard deviation.