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Binding competition experiments shows that Manduca sexta BBMV have lower Cyt1Aa binding sites than Aedes aegypti BBMV. M. sexta or A. aegypti BBMV were incubated with different molar excess of unlabelled and biotin labeled Cyt1Aa was then bound to BBMV samples. BBMV were obtained by centrifugation and separated by SDS-PAGE. Bound biotin-Cyt1Aa was revealed with streptavidin coupled to peroxidase as indicated in Materials and Methods.
