Table 2. Chromatin immunoprecipitation in mouse left ventricle shows specific interaction of EZH2 at genes with bi-directional transcription.
| Ink4a, Ink4b, Ak148321/ANRIL |
| Pax6, Pax6ost1 |
| Nppa, Nppa-as1 |
| Miat, 1700028D13Rik |
| α-MHC, β-MHC, AS β-MHC |
| Foxd2, 9130206I24Rik |
| Hoxc11, Hoxc12, Hotair |
| Gata3, 4930412O13Rik |
| Dio3, Dio3os |
| Ucn, Ucn-as |
| Islr2, 1600029o15Rik |
| Dll4, Gm14207 |
| Pou3f3, 2610017I09Rik |
| 2610100L16Rik, Gm10724 |
| Hoxa4, Hoxa5, Hoxa6, Hoxa7, 2700086A05Rik |
| Irx5, 4933436c20Rik |
| Fbxo44, Fbxo2 |
| Otx2, Otx2os |
| H2-K2, AA388235 |
| Pcnxl2, Bc021891 |
| Dlx6, Dlx6as-1 |
| Tbx2, 2610027K06Rik |
| Myl4 (ALC-1), Myl4-AS |
| cTn1 (Tnnt3), cTn1-AS |
| Tgfβ3, Tgfβ3-AS |
Listed are genes as enriched by ChIP using antibodies that recognize EZH2 and H3K27me3 modification.52 Genes on sense and antisense strands are distinguished by an underline. Significant proportion of the genes enriched by EZH2-ChIP in the mouse heart show specific binding of EZH2 at key cardiac genes with antisense RNA expression. Several cardiac genes with antisense RNA expression including the cardiac regulatory lncRNA genes ANRIL, MIAT, and NPPA-AS appear to be bound by EZH2. Genes encoding non-cardiomyocyte expression programs such as the PAX6, which expresses opposite strand transcript is also repressed by direct binding of EZH2 in the heart. Increased expression of Myosin light chain (MYL4) and TGFβ-3 genes was observed in EZH2 deficient mice,10,52 both of which are known to express regulatory antisense transcripts, however, show no direct association of EZH2 at these promoters.52