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. Author manuscript; available in PMC: 2014 Mar 3.
Published in final edited form as: Circulation. 2012 Oct 3;126(15):1882–1895. doi: 10.1161/CIRCULATIONAHA.112.113290

Figure 2. Expression of CXCL12 isoforms in ischemic tissue.

Figure 2

(A) Quantitative evaluation and relative levels of CXCL12α, CXCL12β and CXCL12γ mRNA contents 1, 4, 6 and 21 days after ischemia in ischemic and non ischemic legs of Cxcl12Gagtm/Gagtm (MUT) and wild-type littermate (WT) animals. Values are mean ± SEM. n=10/group, representative of 2 independent experiments. 28 possible comparisons for Bonferroni correction, a value of p<0.0018 was considered significant, **p<0.0003 versus N Isch at day 1. (B) mRNA levels of each Cxcl12 isoform were normalized to that of Cxcl12α in skeletal muscle of non ischemic tissue. 6 possible comparisons for Bonferroni correction, a value of p<0.008 was considered significant, n=10 per group. (C–D) Histological sections from ischemic skeletal muscles of WT and MUT animals showing CXCL12 expression in capillary (c) and arteriolar (d) structures. Red staining, alpha smooth muscle actin (alpha smooth muscle cells); green staining, Griffonia Simplicifolia Agglutinin isolectin B4 (endothelial cells); purple staining, CXCL12. Nuclei were stained with DAPI (blue staining). Bars: 10μm. One representative experiment out of six is shown.