Abstract
Direct studies of the amino acid sequence of an Escherichia coli plasmid-coded penicillinase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) are in complete agreement with results derived from the translation of the DNA sequence of a related plasmid, apart from a single amino acid substitution. This penicillinase from a Gram-negative bacterium shows 30-35% identity with functionally similar enzymes from Gram-positive bacteria. This paper should be read in conjunction with the report of the DNA sequence of the gene.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ambler R. P., Meadway R. J. Chemical structure of bacterial penicillinases. Nature. 1969 Apr 5;222(5188):24–26. doi: 10.1038/222024a0. [DOI] [PubMed] [Google Scholar]
- Ambler R. P. The amino acid sequence of Staphylococcus aureus penicillinase. Biochem J. 1975 Nov;151(2):197–218. doi: 10.1042/bj1510197. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ambler R. P., Wynn M. The amino acid sequences of cytochromes c-551 from three species of Pseudomonas. Biochem J. 1973 Mar;131(3):485–498. doi: 10.1042/bj1310485. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Datta N., Kontomichalou P. Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae. Nature. 1965 Oct 16;208(5007):239–241. doi: 10.1038/208239a0. [DOI] [PubMed] [Google Scholar]
- Edman P., Begg G. A protein sequenator. Eur J Biochem. 1967 Mar;1(1):80–91. doi: 10.1007/978-3-662-25813-2_14. [DOI] [PubMed] [Google Scholar]
- Hermodson M. A., Ericsson L. H., Titani K., Neurath H., Walsh K. A. Application of sequenator analyses to the study of proteins. Biochemistry. 1972 Nov 21;11(24):4493–4502. doi: 10.1021/bi00774a011. [DOI] [PubMed] [Google Scholar]
- Jack G. W., Richmond M. H. A comparative study of eight distinct beta-lactamases synthesized by gram-negative bacteria. J Gen Microbiol. 1970 Apr;61(1):43–61. doi: 10.1099/00221287-61-1-43. [DOI] [PubMed] [Google Scholar]
- Knox J. R., Kelly J. A., Moews P. C., Murthy N. S. 5-5A crystallographic structure of penicillin beta-lactamase and radius of gyration in solution. J Mol Biol. 1976 Jul 15;104(4):865–875. doi: 10.1016/0022-2836(76)90187-x. [DOI] [PubMed] [Google Scholar]
- Linström E. B., Boman H. G., Steele B. B. Resistance of Escherichia coli to penicillins. VI. Purification and characterization of the chromosomally mediated penicillinase present in ampA-containing strains. J Bacteriol. 1970 Jan;101(1):218–231. doi: 10.1128/jb.101.1.218-231.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matthew M., Hedges R. W. Analytical isoelectric focusing of R factor-determined beta-lactamases: correlation with plasmid compatibility. J Bacteriol. 1976 Feb;125(2):713–718. doi: 10.1128/jb.125.2.713-718.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Melling J., Scott G. K. Preparation of gram quantities of a purified R-factor-mediated penicillinase from Escherichia coli strain W3310. Biochem J. 1972 Nov;130(1):55–62. doi: 10.1042/bj1300055. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Richmond M. H., Sykes R. B. The beta-lactamases of gram-negative bacteria and their possible physiological role. Adv Microb Physiol. 1973;9:31–88. doi: 10.1016/s0065-2911(08)60376-8. [DOI] [PubMed] [Google Scholar]
- Sutcliffe J. G. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737–3741. doi: 10.1073/pnas.75.8.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thatcher D. R. The partial amino acid sequence of the extracellular beta-lactamase I of Bacillus cereus 569/H. Biochem J. 1975 May;147(2):313–326. doi: 10.1042/bj1470313. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamamoto S., Lampen J. O. Membrane penicillinase of Bacillus licheniformis 749/C, a phospholipoprotein. J Biol Chem. 1975 Apr 25;250(8):3212–3213. [PubMed] [Google Scholar]