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. 1978 Aug;75(8):3742–3746. doi: 10.1073/pnas.75.8.3742

High-resolution 13C nuclear magnetic resonance studies of glucose metabolism in Escherichia coli.

K Ugurbil, T R Brown, J A den Hollander, P Glynn, R G Shulman
PMCID: PMC392862  PMID: 358201

Abstract

High-resolution 13C nuclear magnetic resonance spectra of suspensions of Escherichia coli cells have been obtained at 90.5 MHz by using the Fourier transform mode. Anaerobic cells incubated with [I-13C]glucose show a time course of glycolysis in which the alpha and beta glucose anomers disappear at different rates, lactate, succinate, acetate, alanine, and valine accumulate as end products of glycolysis, and fructose bisphosphate appears as an intermediate. It is shown that fructose bisphosphate is labeled at C-1 and C-6 during [I-13C]-glucose catabolism. Upon oxygenation, glutamate appears with the 13C ENRICHMENT AT THE C-4, C-3, and C-2 positions, with the C-4 most intense. From the position of the 13C label we conclude that valine is formed by condensation of pyruvate and that carbon enters the tricarboxylic acid cycle mainly through acetyl CoA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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