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. 1978 Aug;75(8):3791–3795. doi: 10.1073/pnas.75.8.3791

Transcription patterns of amplified Dytiscus genes coding for ribosomal RNA after injection into Xenopus oocyte nuclei.

M F Trendelenburg, H Zentgraf, W W Franke, J B Gurdon
PMCID: PMC392872  PMID: 278989

Abstract

Oocytes of the frog Xenopus were injected with purified circular DNA containing amplified rRNA genes of the water beetle Dytiscus. Nuclear contents of injected oocytes were spread and examined by electron microscopy. Most of the Dytiscus DNA seen in injected nuclei contained regions indensely packed with polymerases and nascent transcripts. Apparently normal, as well as abnormal, patterns of transcription were observed. By this type of experiment, it may become possible to recognize the transcribed regions and immediate transcripts of cloned DNA molecules whose activity cannot be seen by electron microscopy of normal nuclei.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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