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. 1978 Aug;75(8):3953–3956. doi: 10.1073/pnas.75.8.3953

Murine Ia and human DR antigens: homology of amino-terminal sequences.

J P Allison, L E Walker, W A Russell, M A Pellegrino, S Ferrone, R A Reisfeld, J A Frelinger, J Silver
PMCID: PMC392907  PMID: 99745

Abstract

Murine Ia and human DR antigens were isolated and purified by immunoprecipitation and sodium dodecyl sulfate/polyacrylamide gel electrophoresis with allo- and xenoantisera, respectively. The I-A subregion antigen consists of two chains, designated Aalpha and Abeta, with molecular weights of 35,000 and 26,000, respectively. The I-C subregion antigen likewise consists of two chains, designated Calpha and Cbeta, with molecular weights of 32,000 and 29,000, respectively. Under nonreducing conditions, the Cbeta chain migrates appreciably more rapidly on sodium dodecyl sulfate/polyacrylamide gels than the reduced Cbeta chain, reflecting the presence of an intrachain disulfide bond. The human DR antigen is also a two-chain unit and contains DRalpha and DRbeta components with molecular weights of 34,000 and 28,000, respectively. The DRbeta chain migrates more rapidly before reduction than afterward, like the murine Cbeta chain. The DRbeta and Cbeta chains are also strikingly homologous if a single amino acid shift is imposed on one of those chains. Thus, human DR antigens strongly resemble the murine I-C subregion antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach M. L., Widmer M. B., Bach F. H., Klein J. Mixed leukocyte cultures and immune response region disparity. Transplant Proc. 1973 Mar;5(1):369–375. [PubMed] [Google Scholar]
  2. Cresswell P., Geler S. S. Antisera to human B-lymphocyte membrane glycoproteins block stimulation in mixed lymphocyte culture. Nature. 1975 Sep 11;257(5522):147–149. doi: 10.1038/257147a0. [DOI] [PubMed] [Google Scholar]
  3. Cullen S. E., Freed J. H., Nathenson S. G. Structural and serological properties of murine Ia alloantigens. Transplant Rev. 1976;30:236–270. doi: 10.1111/j.1600-065x.1976.tb00222.x. [DOI] [PubMed] [Google Scholar]
  4. Erb P., Feldmann M. The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens. J Exp Med. 1975 Aug 1;142(2):460–472. doi: 10.1084/jem.142.2.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ferrone S., Pellegrino M. A., Reisfeld R. A. Immunogenicity of human B cell antigens solubilized from cultured human lymphoid cells. J Immunol. 1977 Mar;118(3):1036–1041. [PubMed] [Google Scholar]
  6. Hall R. E., Colten H. R. Cell-free synthesis of the fourth component of guinea pig complement (C4): identification of a precursor of serum C4 (pro-C4). Proc Natl Acad Sci U S A. 1977 Apr;74(4):1707–1710. doi: 10.1073/pnas.74.4.1707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Humphreys R. E., McCune J. M., Chess L., Herrman H. C., Malenka D. J., Mann D. L., Parham P., Schlossman S. F., Strominger J. L. Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen. J Exp Med. 1976 Jul 1;144(1):98–112. doi: 10.1084/jem.144.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. McMillan M., Cecka J. M., Murphy D. B., McDevitt H. O., Hood L. Structure of murine Ia antigens: partial NH2-terminal amino acid sequences of products of the I-E or I-C subregion. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5135–5139. doi: 10.1073/pnas.74.11.5135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schwartz B. D., Paul W. E., Shevach E. M. Guinea-pig Ia antigens: functional significance and chemical characterization. Transplant Rev. 1976;30:174–196. doi: 10.1111/j.1600-065x.1976.tb00220.x. [DOI] [PubMed] [Google Scholar]
  12. Shreffler D. C., David C. S., Cullen S. E., Frelinger J. A., Niederhuber J. E. Serological and functional evidence for further subdivision of the I regions of the H-2 gene complex. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):477–487. doi: 10.1101/sqb.1977.041.01.055. [DOI] [PubMed] [Google Scholar]
  13. Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
  14. Silver J., Cecka J. M., McMillan M., Hood L. Chemical characterization of products of the H-2 complex. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):369–377. doi: 10.1101/sqb.1977.041.01.044. [DOI] [PubMed] [Google Scholar]
  15. Silver J. Partial amino acid sequences of Ia alloantigens. Transplant Proc. 1977 Mar;9(1):661–665. [PubMed] [Google Scholar]
  16. Silver J., Russell W. A., Reis B. L., Frelinger J. A. Chemical characterization of murine Ia alloantigens determined by the i-E/i-C subregions of the H-2 complex. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5131–5134. doi: 10.1073/pnas.74.11.5131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Snary D., Barnstable C. J., Bodmer W. F., Goodfellow P. N., Crumpton M. J. Cellular distrubtion, purification, and molecular nature of human Ia antigens. Scand J Immunol. 1977;6(5):439–452. doi: 10.1111/j.1365-3083.1977.tb02101.x. [DOI] [PubMed] [Google Scholar]
  18. Springer T. A., Kaufman J. F., Terhorst C., Strominger J. L. Purification and structural characterisation of human HLA-linked B-cell antigens. Nature. 1977 Jul 21;268(5617):213–218. doi: 10.1038/268213a0. [DOI] [PubMed] [Google Scholar]
  19. Strominger J. L., Ferguson W., Fuks A., Giphart M., Kaufman J., Mann D., Orr H., Parham P., Robb R., Terhorst C. Isolation and structure of HLA antigens. Birth Defects Orig Artic Ser. 1978;14(2):235–246. [PubMed] [Google Scholar]
  20. Walford R. L., Gossett T., Smith G. S., Zeller E., Wilkinson J. A new alloantigenic system on human lymphocytes. Tissue Antigens. 1975 Apr;5(3):196–204. doi: 10.1111/j.1399-0039.1975.tb01214.x. [DOI] [PubMed] [Google Scholar]
  21. Winchester R. J., Wernet P., Kunkel H. G., Dupont B., Jersild C., Fu S. M. Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes. J Exp Med. 1975 Apr 1;141(4):924–929. [PMC free article] [PubMed] [Google Scholar]
  22. Yunis E. J., Amos D. B. Three closely linked genetic systems relevant to transplantation. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3031–3035. doi: 10.1073/pnas.68.12.3031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van Rood J. J., Koch C. T., van Hooff J. P., van Leeuwen A., van den Tweel J. G., Frederiks E., Schippers H. M., Hendriks G., van der Steen G. J. Graft survival in unrelated donor--recipient pairs matched for MLC and HL-A. Transplant Proc. 1973 Mar;5(1):409–414. [PubMed] [Google Scholar]

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