Abstract
Striatal extracts of guinea pigs, rats, and cattle were found to contain two large proteins (greater than 40,000 and greater than 100,000 daltons) that on treatment with trypsin yield opioid peptides differing chromatographically from the opioid nonapeptide generated by trypsin digestion of endorphins, beta-lipotropin, or pro-opiocortin. Furthermore, the large opioid proteins found in the pituitary do not appear to be present in the striatum. These and other findings indicate that the striatal enkephalins are produced via a pathway from the one deduced from studies on the pituitary.
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- Böhlen P., Stein S., Stone J., Udenfriend S. Automatic Monitoring of primary amines in preparative column effluents with fluorescamine. Anal Biochem. 1975 Aug;67(2):438–445. doi: 10.1016/0003-2697(75)90316-4. [DOI] [PubMed] [Google Scholar]
- Denckla W. D. Minimal O 2 consumption as an index of thyroid status: standardization of method. Endocrinology. 1973 Jul;93(1):61–73. doi: 10.1210/endo-93-1-61. [DOI] [PubMed] [Google Scholar]
- Hughes J., Smith T. W., Kosterlitz H. W., Fothergill L. A., Morgan B. A., Morris H. R. Identification of two related pentapeptides from the brain with potent opiate agonist activity. Nature. 1975 Dec 18;258(5536):577–580. doi: 10.1038/258577a0. [DOI] [PubMed] [Google Scholar]
- Lazarus L. H., Ling N., Guillemin R. beta-Lipotropin as a prohormone for the morphinomimetic peptides endorphins and enkephalins. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2156–2159. doi: 10.1073/pnas.73.6.2156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mains R. E., Eipper B. A. Coordinate synthesis of corticotropins and endorphins by mouse pituitary tumor cells. J Biol Chem. 1978 Feb 10;253(3):651–655. [PubMed] [Google Scholar]
- Mains R. E., Eipper B. A., Ling N. Common precursor to corticotropins and endorphins. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3014–3018. doi: 10.1073/pnas.74.7.3014. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pert A., Simantov R., Snyder S. H. A morphine-like factor in mammalian brain: analgesic activity in rats. Brain Res. 1977 Nov 18;136(3):523–533. doi: 10.1016/0006-8993(77)90076-2. [DOI] [PubMed] [Google Scholar]
- Rubinstein M., Stein S., Gerber L. D., Udenfriend S. Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3052–3055. doi: 10.1073/pnas.74.7.3052. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubinstein M., Stein S., Udenfriend S. Characterization of pro-opiocortin, a precursor to opioid peptides and corticotropin. Proc Natl Acad Sci U S A. 1978 Feb;75(2):669–671. doi: 10.1073/pnas.75.2.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubinstein M., Stein S., Udenfriend S. Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4969–4972. doi: 10.1073/pnas.74.11.4969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simantov R., Snyder S. H. Morphine-like peptides in mammalian brain: isolation, structure elucidation, and interactions with the opiate receptor. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2515–2519. doi: 10.1073/pnas.73.7.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]