Abstract
We report magnetic circular dichroism (MCD) spectra of 4-Fe iron-sulfur clusters in the iron-sulfur proteins Chromatium high-potential iron protein (HIPIP), Bacillus stearothermophilus ferredoxin and Clostridium pasteurianum ferredoxin. The MCD is found to vary significantly with cluster oxidation state but is relatively insensitive to the nature of the protein. The spectra obtained are compared with the corresponding spectra of iron-sulfur proteins containing 2-Fe clusters. It is concluded that MCD is useful for the characterization of iron-sulfur cluster type and oxidation state in iron-sulfur proteins and is superior for this purpose to absorption and natural circular dichroism spectroscopy.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cerdonio M., Wang R. H., Rawlings J., Gray H. B. Letter: Spectroscopic and magnetic characterization of high potential iron-sulfur protein from Chromatium. J Am Chem Soc. 1974 Oct 2;96(20):6534–6535. doi: 10.1021/ja00827a057. [DOI] [PubMed] [Google Scholar]
- Eaton W. A., Palmer G., Fee J. A., Kimura T., Lovenberg W. Tetrahedral iron in the active center of plant ferredoxins and beef adrenodoxin. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3015–3020. doi: 10.1073/pnas.68.12.3015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hill C. L., Steenkamp D. J., Holm R. H., Singer T. P. Identification of the iron-sulfur center in trimethylamine dehydrogenase. Proc Natl Acad Sci U S A. 1977 Feb;74(2):547–551. doi: 10.1073/pnas.74.2.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holm R. H., Averill B. A., Herskovitz T., Frankel R. B., Gray H. B., Siiman O., Grunthaner F. J. Equivalence of metal centers in the iron-sulfur protein active site analogs (Fe4S4(SR)4)2-. J Am Chem Soc. 1974 Apr 17;96(8):2644–2646. doi: 10.1021/ja00815a071. [DOI] [PubMed] [Google Scholar]
- Rivoal J. C., Briat B., Cammack R., Hall D. O., Rao K. K., Douglas I. N., Thomson A. J. The low temperature magnetic circular dichroism spectra of iron-sulphur proteins. I. Oxidised rubredoxin. Biochim Biophys Acta. 1977 Jul 22;493(1):122–131. doi: 10.1016/0005-2795(77)90265-3. [DOI] [PubMed] [Google Scholar]
- Sutherland J. C., Salmeen I., Sun A. S., Klein M. P. Ferredoxin: the uses of natural and magnetic circular dichroism in an multi-chromophoric system. Biochim Biophys Acta. 1972 May 18;263(3):550–554. doi: 10.1016/0005-2795(72)90035-9. [DOI] [PubMed] [Google Scholar]
- Thomson A. J., Cammack R., Hall D. O., Rao K. K., Briat B., Rivoal J. C., Badoz J. The low temperature magnetic circular dichroism spectra of iron-sulphur proteins. II. Two-iron ferredoxins. Biochim Biophys Acta. 1977 Jul 22;493(1):132–141. doi: 10.1016/0005-2795(77)90266-5. [DOI] [PubMed] [Google Scholar]
- Ulmer D. D., Holmquist B., Vallee B. L. Magnetic circular dichroism of non-heme iron proteins. Biochem Biophys Res Commun. 1973 Apr 16;51(4):1054–1061. doi: 10.1016/0006-291x(73)90034-x. [DOI] [PubMed] [Google Scholar]