Figure 7. Surface plasmon resonance (SPR) based affinity ranking for IgGs converted from affinity matured SH313-F9 variants. IgGs converted from scFv (A), scFabΔC (B), or FabΔC (C) affinity matured candidates were captured on a CM5 chip modified with a mouse anti-IgG Fc specific capture antibody and probed with monomeric CD30. (D) Summary of apparent affinity constants.