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. 1978 Nov;75(11):5281–5285. doi: 10.1073/pnas.75.11.5281

A photo-CIDNP study of the interaction of oligonucleotides with gene-5 protein of bacteriophage M13.

G J Garssen, R Kaptein, J G Schoenmakers, C W Hilbers
PMCID: PMC392946  PMID: 364473

Abstract

It is shown that photo-CIDNP effects (CIDNP, chemically induced dynamic nuclear polarization) can be generated in the 360-MHz proton NMR spectrum of gene-5 protein from bacteriophage M13. This technique is used to determine the number of tyrosyl residues at the surface of the protein and to assign the resonances from the 3,5-ring protons of these residues. The DNA-binding site of the protein is investigated by formation of complexes with oligonucleotides. Complex formation leads to shifting and/or quenching of the photo-CIDNP emission signals of the surface tyrosines, implying that they are involved in DNA-protein interaction. These experiments are complemented by studying the complex formation of Lys-Tyr-Lys to poly(A).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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