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A, Western blot analysis shows that mice fed an HFD have decreased p-AMPK and p-ACC levels compared with mice on an NCD with or without nicotine treatment. Addition of nicotine to HFD leads to complete dephosphorylation of AMPK and ACC. B, Treatment with acipimox significantly attenuates the HFD plus nicotine-induced decrease in p-AMPK and p-ACC levels.
