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. 1978 Nov;75(11):5462–5465. doi: 10.1073/pnas.75.11.5462

Sequence of oxygen binding by hemoglobin

Toshio Asakura 1,2, Pui-wah Lau 1,2
PMCID: PMC392984  PMID: 214788

Abstract

A nitroxide spin-label probe was attached directly to a propionic acid group of heme in either the α or the β chain of hemoglobin. The electron paramagnetic resonance (EPR) spectrum of the spin label is altered by the spin-state change of the heme iron to which the spin label is attached. These hybrid hemoglobins showed normal optical and functional properties, indicating that the attachment of the spin label did not perturb the function of hemoglobin. Upon deoxygenation of α-heme-spin-labeled hemoglobin, EPR signals changed proportionally with oxygen saturation (determined by measuring absorption spectra). This result indicates that there is no binding preference between the α and β chains of hemoglobin. However, the cross plot for the fraction of the EPR changes vs. the fraction of oxygen saturation deviated significantly from the diagonal straight line in response to the addition of 2,3-diphosphoglycerate and inositol hexaphosphate. The deviation indicated that the EPR change precedes the optical change at low oxygen tension. This result implies that, in the presence of organic phosphate, oxygen binds preferentially to the α subunit of deoxyhemoglobin. This conclusion was supported by the result obtained with β-heme-spin-labeled hemoglobin: the direction of the deviation for β-heme-spin-labeled hemoglobin in the presence of diphosphoglycerate and inositol hexaphosphate was opposite to that obtained for α-heme-spin-labeled hemoglobin. However, the curve deviated even in the absence of organic phosphate. This deviation for β-heme-spin-labeled hemoglobin can be explained by the intersubunit interaction of hemoglobin. From these results, it was concluded that, in the absence of organic phosphate, oxygen combines with the α and β chains with equal probability whereas, in the presence of organic phosphate, oxygen binds preferentially to the α chains of hemoglobin.

Keywords: hemoglobin subunits, spin-labeling, electron paramagnetic resonance, heme-heme interactions

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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