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. 1978 Dec;75(12):5851–5854. doi: 10.1073/pnas.75.12.5851

An amino-terminal fragment of lac repressor binds specifically to lac operator

Ronald T Ogata 1, Walter Gilbert 1
PMCID: PMC393073  PMID: 16592594

Abstract

We have examined the effect of an aminoterminal peptide from the lac repressor (residues 1-59) on the methylation of purines in the lac operator with dimethyl sulfate. The peptide perturbs the methylation of the operator, and the peptide-induced pattern of inhibition and enhancement of methylation, across the operator, closely resembles the pattern induced by intact repressor. This demonstrates that this small amino-terminal peptide binds specifically to the lac operator and that the mechanism of recognition and binding is basically the same as that of intact repressor.

Keywords: dimethyl sulfate methylation, tryptic peptide, bromouracil DNA, protein domains

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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