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. 1978 Dec;75(12):5860–5863. doi: 10.1073/pnas.75.12.5860

Structure of the oxygen adduct intermediate in the bacterial luciferase reaction: 13C nuclear magnetic resonance determination*

Sandro Ghisla †, J Woodland Hastings ‡, Vincent Favaudon §, Jean-Marc Lhoste §
PMCID: PMC393075  PMID: 16592595

Abstract

By using FMN enriched in 13C (90%) at position C-4a, we have conclusively shown that the reaction of molecular oxygen with bacterial luciferase-bound FMNH2 forms an adduct at the 4a position. Consistent with this are 13C NMR studies of FMN and other flavin compounds which show that this carbon should be unusually reactive in the reduced 1,5-dihydroflavins with respect to electrophilic attacks.

Keywords: subzero temperature, oxidation-reduction, substituted flavins, flavoproteins

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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