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. 1978 Dec;75(12):5912–5916. doi: 10.1073/pnas.75.12.5912

Isolation and characterization of a tumor cell surface antigen from spontaneously transformed BALB/c mouse fibroblasts.

A R Kamm, W J Grimes
PMCID: PMC393086  PMID: 282613

Abstract

This work describes the detection, isolation, and partial characterization of a BALB/c mouse fibroblast cell surface antigen. This antigen migrates as a polypeptide of approximately 100,000 daltons in a discontinuous sodium dodecyl sulfate/polyacrylamide gel electrophoresis system, can be labeled by either lactoperoxidase-catalyzed cell surface 125I iodination or metabolic incorporation of [3H]glucosamine, and can be isolated by concanavalin A affinity chromatography. This cell surface glycoprotein is antigenic in BALB/c mice and has been correlated with the rejection of immunogenic tumor cells. Also, antiserum specific for Moloney leukemia virus precipitates the 100,000-dalton cell surface protein from viral and immunogenic spontaneous transformants. This virus-related antigen comigrates on sodium dodecyl sulfate gels with the major iodinated cell surface protein of these transformants. Rabbit antiserum to the purified antigen demonstrates a marked preference for the surfaces of immunogenic tumor cells as compared with normal cells and nonimmunogenic tumor cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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