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. 1978 Dec;75(12):6035–6038. doi: 10.1073/pnas.75.12.6035

Demonstration of an ecdysteroid receptor in a cultured cell line of Drosophila melanogaster.

P Maroy, R Dennis, C Beckers, B A Sage, J D O'Connor
PMCID: PMC393112  PMID: 216008

Abstract

Incubation of the high-speed supernatant from the Kc cell line of Drosophila melanogaster with [3H]ponasterone A results in significant binding of the ligand as determined by gel filtration and dextran-coated charcoal binding assays. In vivo exposure of the Kc line to [3H]ponasterone A for 30 min results in a marked binding of the ligand by a KCl-soluble nuclear extract. With both the cytosol and nuclear preparations the binding has specificity and a low dissociation constant (3 X 10(-9) M). In addition the labeling of the nuclear preparation exhibits a saturation at approximately 7 X 10(-10) M which probably reflects the molar concentration of cytoplasmic receptors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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