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. 1978 Dec;75(12):6069–6072. doi: 10.1073/pnas.75.12.6069

Loss of proliferative calcium dependence: simple in vitro indicator of tumorigenicity.

S H Swierenga, J F Whitfield, S Karasaki
PMCID: PMC393119  PMID: 282624

Abstract

The proliferative activities of three lines of "normal" epithelioid rat liver cells and six tumorigenic liver cell lines in the presence of a wide range of calcium concentrations were measured by a simple colony forming assay. The proliferative activities of the normal cells and, to a lesser extent, of the cells of a marginally tumorigenic line were directly proportional to the extracellular calcium concentration. The proliferative activities of the cells of the strongly tumorigenic lines, on the other hand, were either uninfluenced by or inversely proportional to the extracellular calcium concentration. Thus, the proliferative response to the extracellular calcium concentration is a sensitive indicator of the carcinogenic potential of liver cells.

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Selected References

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  1. Balk S. D., Whitfield J. F., Youdale T., Braun A. C. Roles of calcium, serum, plasma, and folic acid in the control of proliferation of normal and Rous sarcoma virus-infected chicken fibroblasts. Proc Natl Acad Sci U S A. 1973 Mar;70(3):675–679. doi: 10.1073/pnas.70.3.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boynton A. L., Whitfield J. F. Calcium requirements for the proliferation of cells infected with a temperature-sensitive mutant of Rous sarcoma virus. Cancer Res. 1978 May;38(5):1237–1240. [PubMed] [Google Scholar]
  3. Boynton A. L., Whitfield J. F. Different calcium requirements for proliferation of conditionally and unconditionally tumorigenic mouse cells. Proc Natl Acad Sci U S A. 1976 May;73(5):1651–1654. doi: 10.1073/pnas.73.5.1651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boynton A. L., Whitfield J. F., Isaacs R. J., Tremblay R. G. Different extracellular calcium requirements for proliferation of nonneoplastic, preneoplastic, and neoplastic mouse cells. Cancer Res. 1977 Aug;37(8 Pt 1):2657–2661. [PubMed] [Google Scholar]
  5. Boynton A. L., Whitfield J. F., Isaacs R. J., Tremblay R. The control of human WI-38 cell proliferation by extracellular calcium and its elimination by SV-40 virus-induced proliferative transformation. J Cell Physiol. 1977 Aug;92(2):241–247. doi: 10.1002/jcp.1040920212. [DOI] [PubMed] [Google Scholar]
  6. Chessebeuf M., Olsson A., Bournot P., Desgres J., Guiguet M., Maume G., Maume B. F., Perissel B., Padieu P. Long term cell culture of rat liver epithelial cells retaining some hepatic functions. Biochimie. 1974;56(10):1365–1379. doi: 10.1016/s0300-9084(75)80023-x. [DOI] [PubMed] [Google Scholar]
  7. Freeman A. E., Black P. H., Wolford R., Huebner R. J. Adenovirus type 12-rat embryo transformation system. J Virol. 1967 Apr;1(2):362–367. doi: 10.1128/jvi.1.2.362-367.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Karasaki S., Okigaki T. Surface membrane nucleoside triphosphatase activity and tumorigenicity of cultured liver epithelial cells. Cancer Res. 1976 Dec;36(12):4491–4499. [PubMed] [Google Scholar]
  9. Karasaki S., Simard A., De Lamirande G. Ultrastructural analysis of malignant conversion in vitro of epithlial cells derived from normal adult rat liver. Eur J Cancer. 1976 Jul;12(7):527–534. doi: 10.1016/0014-2964(76)90158-4. [DOI] [PubMed] [Google Scholar]
  10. Karasaki S., Simard A., de Lamirande G. Surface morphology and nucleoside phosphatase activity of rat liver epithelial cells during oncogenic transformation in vitro. Cancer Res. 1977 Oct;37(10):3516–3525. [PubMed] [Google Scholar]
  11. Parsons P. G. Selective proliferation of human tumour cells in calcium-depleted medium. Aust J Exp Biol Med Sci. 1978 Jun;56(3):297–300. doi: 10.1038/icb.1978.31. [DOI] [PubMed] [Google Scholar]
  12. Rixon R. H., Whitfield J. F. The control of liver regeneration by parathyroid hormone and calcium. J Cell Physiol. 1975 Dec;87(2):147–155. doi: 10.1002/jcp.1040870203. [DOI] [PubMed] [Google Scholar]
  13. San R. H., Williams G. M. Rat hepatocyte primary cell culture-mediated mutagenesis of adult rat liver epithelial cells by procarcinogens. Proc Soc Exp Biol Med. 1977 Dec;156(3):534–538. doi: 10.3181/00379727-156-39974. [DOI] [PubMed] [Google Scholar]
  14. Swierenga S. H., MacManus J. P., Whitfield J. F. Regulation by calcium of the proliferation of heart cells from young adult rats. In Vitro. 1976 Jan;12(1):31–36. doi: 10.1007/BF02832790. [DOI] [PubMed] [Google Scholar]
  15. Swierenga S. H., Whitfield J. F., Gillan D. J. Alteration by malignant transformation of the calcium requirements for cell proliferation in vitro. J Natl Cancer Inst. 1976 Jul;57(1):125–129. doi: 10.1093/jnci/57.1.125. [DOI] [PubMed] [Google Scholar]
  16. Swierenga S. H., Whitfield J. F., Morris H. P. The reduced extracellular calcium requirement for proliferation by neoplastic hepatocytes. In Vitro. 1978 Jun;14(6):527–535. doi: 10.1007/BF02616095. [DOI] [PubMed] [Google Scholar]
  17. Whitfield J. F., MacManus J. P., Rixon R. H., Boynton A. L., Youdale T., Swierenga S. The positive control of cell proliferation by the interplay on calcium ions and cyclic nucleotides. A review. In Vitro. 1976 Jan;12(1):1–18. doi: 10.1007/BF02832787. [DOI] [PubMed] [Google Scholar]
  18. Yang G. C., Brubaker R. R. Effect of ca on the synthesis of deoxyribonucleic Acid in virulent and avirulent yersinia. Infect Immun. 1971 Jan;3(1):59–65. doi: 10.1128/iai.3.1.59-65.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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